Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD279 PE-Cy™7

Anti-Hu CD279 PE-Cy™7

Regulatory status

RUO

Antigen

CD279

Clone

EH12.2H7

Format

PE-Cy™7

Reactivity

Non-human primates, Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

T7-176-T100

In stock

275.00 USD

Variant

100 tests

T7-176-T100

In stock

275.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody EH12.2H7 recognizes an extracellular epitope of CD279 / PD-1 (programmed cell death 1), a 55 kDa type I transmembrane protein expressed above all during T cell development, on activated T cells, activated B cells, and activated monocytes.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Non-human primates, Human

Immunogen

recombinant human CD279

Preparation

Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy™7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Cy™ and CyDye™ are registered trademarks of Cytiva.

Other names

PDCD1, PD1, SLEB2

Antigen description

CD279 / PD-1 (programmed cell death 1), a transmembrane protein of CD28/CTLA-4 family. It is expressed inducibly mainly on activated T, B, and myeloid cells and plays a role in maintaining peripheral self-tolerance. Binding to its ligands CD273 and CD274 is associated with inhibition of T cell proliferation and induction of their anergy. It is also expressed during thymic development. Some variants of CD279 are associated with susceptibility to systemic lupus erythematosus, type 1 diabetes, and rheumatoid arthritis.

Entrez Gene ID 5133

UniProt ID Q15116

Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononuklear cells stained using anti-human CD279 (EH12.2H7) PE-Cy™7 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Separation of human PHA stimulated peripheral blood mononuklear cells stained using anti-human CD279 (EH12.2H7) PE-Cy™7 antibody (4 μl reagent per milion cells in 100 μl of cell suspension, red-filled) from human PHA stimulated peripheral blood mononuklear cells stained using mouse IgG1 isotype control (MOPC-21) PE-Cy™7 antibody (concentration in sample 1,67 μg/ml, same as CD279 PE-Cy™7 concentration, black-dashed) in flow cytometry analysis (surface staining).

Product specific references:

Xu Y, Weatherall C, Bailey M, Alcantara S, De Rose R, Estaquier J, Wilson K, Suzuki K, Corbeil J, Cooper DA, Kent SJ, Kelleher AD, Zaunders J: Simian immunodeficiency virus infects follicular helper CD4 T cells in lymphoid tissues during pathogenic infection of pigtail macaques. J Virol. 2013 Apr;87(7):3760-73.
PubMed

Bedoret D, Singh AK, Shaw V, Hoyte EG, Hamilton R, DeKruyff RH, Schneider LC, Nadeau KC, Umetsu DT: Changes in antigen-specific T-cell number and function during oral desensitization in cow's milk allergy enabled with omalizumab. Mucosal Immunol. 2012 May;5(3):267-76.
PubMed

Gros A, Robbins PF, Yao X, Li YF, Turcotte S, Tran E, Wunderlich JR, Mixon A, Farid S, Dudley ME, Hanada K, Almeida JR, Darko S, Douek DC, Yang JC, Rosenberg SA: PD-1 identifies the patient-specific CD8⁺ tumor-reactive repertoire infiltrating human tumors. J Clin Invest. 2014 May;124(5):2246-59.
PubMed

Haile ST, Dalal SP, Clements V, Tamada K, Ostrand-Rosenberg S: Soluble CD80 restores T cell activation and overcomes tumor cell programmed death ligand 1-mediated immune suppression. J Immunol. 2013 Sep 1;191(5):2829-36.
PubMed

Černý V, Hrdý J, Novotná O, Petrásková P, Boráková K, Kolářová L, Prokešová L: Distinct characteristics of Tregs of newborns of healthy and allergic mothers. PLoS One. 2018 Nov 26;13(11):e0207998.
PubMed

Zhou J, Cheung AK, Liu H, Tan Z, Tang X, Kang Y, Du Y, Wang H, Liu L, Chen Z: Potentiating functional antigen-specific CD8⁺ T cell immunity by a novel PD1 isoform-based fusion DNA vaccine. Mol Ther. 2013 Jul;21(7):1445-55.
PubMed

Li H, Pauza CD: CD25(+) Bcl6(low) T follicular helper cells provide help to maturing B cells in germinal centers of human tonsil. Eur J Immunol. 2015 Jan;45(1):298-308.
PubMed

Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Martinez Casals A, Tian H, Lowery L, Neumann EK, Björklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Mueller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Börner K, Radtke AJ: Organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging. Nat Methods 2023 Aug;20(8):1174-1178. doi: 10.1038/s41592-023-01846-7.
PubMed