Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD276 PE

Anti-Hu CD276 PE

Regulatory status

RUO

Antigen

CD276

Clone

7-517

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-963-T100

In stock

248.60 USD

Variant

100 tests

1P-963-T100

In stock

248.60 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody 7-517 recognizes an extracellular epitope of human CD276 (B7H3) transmembrane glycoprotein.

Workshop

HLDA8: WS code DC ARN-80650

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

monocyte-derived dendritic cells

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

B7H3, B7RP-2

Antigen description

CD276 (B7H3) is a type I transmembrane glycoprotein expressed on dendritic cells, osteoblasts, sinonasal epithelium, and upon activation (by inflammatory cytokines) in monocytes, NK cells, T cells and B cells. Although it has been described also as a costimulatory ligand, its major role seems to be inhibitory. It regullates T cell proliferation and functions, and may provide certain tumors with protection against NK cells. CD276 can be used as a marker of neuroblastoma cells in the bone marrow, which is the primary site of neuroblastoma relapse.

Entrez Gene ID 80381

UniProt ID Q5ZPR3

Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) monocytes stained using anti-human CD276 (7-517) PE antibody (concentration in sample 1.67 μg/ml).

Flow cytometry multicolor surface staining pattern of human stimulated (GM-CSF + IL-4) monocytes stained using anti-human CD11c (BU15) APC antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD276 (7-517) PE antibody (concentration in sample 1.67 μg/ml).

Separation of human CD11c positive CD276 positive cells (red-filled) from CD11c negative CD276 negative cells (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) monocytes stained using anti-human CD276 (7-517) PE antibody (concentration in sample 1.67 μg/ml).

Product specific references:

Camilleri ET, Gustafson MP, Dudakovic A, Riester SM, Garces CG, Paradise CR, Takai H, Karperien M, Cool S, Sampen HJ, Larson AN, Qu W, Smith J, Dietz AB, van Wijnen AJ: Identification and validation of multiple cell surface markers of clinical-grade adipose-derived mesenchymal stromal cells as novel release criteria for good manufacturing practice-compliant production. Stem Cell Res Ther. 2016 Aug 11;7(1):107.
PubMed

Yoon BR, Chung YH, Yoo SJ, Kawara K, Kim J, Yoo IS, Park CG, Kang SW, Lee WW: Preferential induction of the T cell auxiliary signaling molecule B7-H3 on synovial monocytes in rheumatoid arthritis. J Biol Chem. 2016 Feb 19;291(8):4048-57.
PubMed

Genova C, Tasso R, Rosa A, Rossi G, Reverberi D, Fontana V, Marconi S, Croce M, Dal Bello MG, Dellepiane C, Tagliamento M, Ciferri MC, Zullo L, Fedeli A, Alama A, Cortese K, Gentili C, Cella E, Anselmi G, Mora M, Barletta G, Rijavec E, Grossi F, Pronzato P, Coco S: Prognostic role of soluble and extracellular vesicle-associated PD-L1, B7-H3 and B7-H4 in non-small cell lung cancer patients treated with immune checkpoint inhibitors. Cells 2023 Mar 8;12(6):832.
PubMed

Du H, Hirabayashi K, Ahn S, Kren NP, Montgomery SA, Wang X, Tiruthani K, Mirlekar B, Michaud D, Greene K, Herrera SG, Xu Y, Sun C, Chen Y, Ma X, Ferrone CR, Pylayeva-Gupta Y, Yeh JJ, Liu R, Savoldo B, Ferrone S, Dotti G: Antitumor responses in the absence of toxicity in solid tumors by targeting B7-H3 via chimeric antigen receptor T cells. Cancer Cell 2019 Feb 11;35(2):221-237.
PubMed

Vogl UM, Öhler L, Rasic M, Frischer JM, Modak M, Stöckl J: Evaluation of prognostic immune signatures in patients with breast, colorectal and pancreatic cancer receiving chemotherapy. Anticancer Res. 2017 Apr;37(4):1947-1955.
PubMed

Nehama D, Di Ianni N, Musio S, Du H, Patané M, Pollo B, Finocchiaro G, Park JJ, Dunn DE, Edwards DS, Damrauer JS, Hudson H, Floyd SR, Ferrone S, Savoldo B, Pellegatta S, Dotti G: B7-H3-redirected chimeric antigen receptor T cells target glioblastoma and neurospheres. EBioMedicine 2019 Sep:47:33-43.
PubMed