Anti-Hu CD273 PE

Anti-Hu CD273 PE
Regulatory status
RUO
Antigen
CD273
Clone
24F.10C12
Format
PE
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1P-178-T100
In stock
220.00 USD

25 tests
1P-178-T025
Delivery 1 week
110.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD

0.1 mg
11-261-C100
In stock
110.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG2a kappa
Specificity
The mouse monoclonal antibody 24F.10C12 recognizes an extracellular epitope of CD273 / PD-L2 (also known as B7-DC), a 25 kDa type I transmembrane protein expressed by dendritic cells, activated monocytes and T cells, heart, first trimester placenta, lung and liver, as well as in Hodgkin´s lymphoma cells and primary mediastinal B cell lymphoma (PMBL).
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
human CD273 cDNA
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
PDCD1LG2, B7DC, Btdc, PDL2, PDCD1L2, bA574F11.2, Butyrophilin, B7-DC,
Antigen description
CD273 / PD-L2 (programmed death ligand-1), also known as B7-DC, is a member of the B7 family of regulatory proteins. It costimulates the proliferation of T cells, and mediates IFN gamma production. Ligation of CD273 on dendritic cells enhances dendritic cell activation and T cell responses. When interacting with CD279, it can act as a coinhibitor of the T cell function. CD273 expression is a useful marker to distinguish primary mediastinal B cell lymphoma from other diffuse large B cell lymphomas.
Entrez Gene ID 80380
UniProt ID Q9BQ51
1P-178_FC_Profil Značení
Flow cytometry surface staining pattern of human GM-CSF and Il-4 stimulated peripheral blood mononuclear cells stained using anti-human CD273 (24F.10C12) PE antibody (10 μl reagent per milion cells in 100 μl of cell suspension).
1P-178_FC_Dot-plot
Flow cytometry multicolor surface staining of human GM-CSF and Il-4 stimulated peripheral blood mononuclear cells stained using anti-human CD273 (24F.10C12) PE antibody (10 μl reagent per milion cells in 100 μl of cell suspension) and anti-human CD11c (BU15) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
1P-178_FC_Histogram
Separation of human CD273 positive CD11c positive cells (red-filled) from CD273 negative CD11c negative cells (black-dashed) in flow cytometry analysis (surface staining) of human GM-CSF and Il-4 stimulated peripheral blood mononuclear cells stained using anti-human CD273 (24F.10C12) PE antibody (10 μl reagent per milion cells in 100 μl of cell suspension).

Product specific references:

Green MR, Monti S, Rodig SJ, Juszczynski P, Currie T, O'Donnell E, Chapuy B, Takeyama K, Neuberg D, Golub TR, Kutok JL, Shipp MA: Integrative analysis reveals selective 9p24.1 amplification, increased PD-1 ligand expression, and further induction via JAK2 in nodular sclerosing Hodgkin lymphoma and primary mediastinal large B-cell lymphoma. Blood. 2010 Oct 28;116(17):3268-77.
PubMed
Rodríguez-García M, Porichis F, de Jong OG, Levi K, Diefenbach TJ, Lifson JD, Freeman GJ, Walker BD, Kaufmann DE, Kavanagh DG: Expression of PD-L1 and PD-L2 on human macrophages is up-regulated by HIV-1 and differentially modulated by IL-10. J Leukoc Biol. 2011 Apr;89(4):507-15.
PubMed
Semnani RT, Mahapatra L, Moore V, Sanprasert V, Nutman TB: Functional and phenotypic characteristics of alternative activation induced in human monocytes by interleukin-4 or the parasitic nematode Brugia malayi. Infect Immun. 2011 Oct;79(10):3957-65.
PubMed
Variant
100 tests
1P-178-T100
In stock
220.00 USD

25 tests
1P-178-T025
Delivery 1 week
110.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD

0.1 mg
11-261-C100
In stock
110.00 USD

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