Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD273 APC

Anti-Hu CD273 APC

Anti-Hu CD273 APC
Regulatory status
RUO
Antigen
CD273
Clone
24F.10C12
Format
APC
Reactivity
Human
Application
FC (QC tested)
Excitation laser
red (633 nm)
Variant
100 tests
1A-178-T100
In stock
Variant
0.1 mg
11-261-C100
In stock
Contact us
Product details
Images
References
Isotype
Mouse IgG2a kappa
Specificity
The mouse monoclonal antibody 24F.10C12 recognizes an extracellular epitope of CD273 / PD-L2 (also known as B7-DC), a 25 kDa type I transmembrane protein expressed by dendritic cells, activated monocytes and T cells, heart, first trimester placenta, lung and liver, as well as in Hodgkin´s lymphoma cells and primary mediastinal B cell lymphoma (PMBL).
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
human CD273
Other names
PDCD1LG2, B7DC, Btdc, PDL2, PD-L2, PDCD1L2, bA574F11.2, Butyrophilin B7-DC, B7-DC
Preparation
The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
1A-178_FC_Profil Značení
Flow cytometry surface staining pattern of human stimulated monocytes (GM-CSF + IL-4) stained using anti-human CD273 (24F.10C12) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).
1A-178_FC_Histogram
Separation of human dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4) (red-filled) from non-stimulated lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human stimulated monocytes (GM-CSF + IL-4) stained using anti-human CD273 (24F.10C12) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Green MR, Monti S, Rodig SJ, Juszczynski P, Currie T, O'Donnell E, Chapuy B, Takeyama K, Neuberg D, Golub TR, Kutok JL, Shipp MA: Integrative analysis reveals selective 9p24.1 amplification, increased PD-1 ligand expression, and further induction via JAK2 in nodular sclerosing Hodgkin lymphoma and primary mediastinal large B-cell lymphoma. Blood. 2010 Oct 28;116(17):3268-77.
PubMed
Rodríguez-García M, Porichis F, de Jong OG, Levi K, Diefenbach TJ, Lifson JD, Freeman GJ, Walker BD, Kaufmann DE, Kavanagh DG: Expression of PD-L1 and PD-L2 on human macrophages is up-regulated by HIV-1 and differentially modulated by IL-10. J Leukoc Biol. 2011 Apr;89(4):507-15.
PubMed
Semnani RT, Mahapatra L, Moore V, Sanprasert V, Nutman TB: Functional and phenotypic characteristics of alternative activation induced in human monocytes by interleukin-4 or the parasitic nematode Brugia malayi. Infect Immun. 2011 Oct;79(10):3957-65.
PubMed
Variant
100 tests
1A-178-T100
In stock
Variant
0.1 mg
11-261-C100
In stock
Contact us
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