Anti-Hu CD272 Purified

Anti-Hu CD272 Purified
Regulatory status
RUO
Antigen
CD272
Clone
MIH26
Format
Purified
Reactivity
Human, Non-human primates
Application
Variant
0.1 mg
11-877-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG2a kappa
Specificity
The mouse monoclonal antibody MIH26 recognizes an extracellular epitope of CD272, a transmembrane glycoprotein serving as a negative regulator of the activation in various leukocyte types.
Application
Application details
Flow cytometry: Recommended dilution: 2-5 μg/ml.
Reactivity
Human, Non-human primates
Immunogen
cells transfected with human CD272
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
BTLA1
Antigen description
CD272, a type I transmembrane glycoprotein, contains in its intracellular domain two ITIM sequences, which are upon CD272 triggering phosphorylated and recruit SHP phosphatases to attenuate cell activation. CD272 is expressed on B and T lymphocytes, NK cells, dendritic cells, and macrophages, and its ligand is CD270. Defects in CD272-CD270 inhibitory mechanism lead to autoimmune diseases. Overexpression of CD272 is a marker of tolerant T cells.
Entrez Gene ID 151888
UniProt ID Q7Z6A9
11-877_FC_Profil značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD272 (MIH26) purified antibody (concentration in sample 1,7 μg/ml, GAM APC).
11-877_FC_Histogram
Separation of human CD272 positive lymphocytes (red-filled) from human neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD272 (MIH26) purified antibody (concentration in sample 1,7 μg/ml, GAM APC).

Product specific references:

Ramsay AG , Clear AJ, Fatah R, Gribben JG: Multiple inhibitory ligands induce impaired T-cell immunologic synapse function in chronic lymphocytic leukemia that can be blocked with lenalidomide: establishing a reversible immune evasion mechanism in human cancer. Blood. 2012 Aug 16;120(7):1412-21.
PubMed
Zhang Z, Xu X, Lu J, Zhang S, Gu L, Fu J, Jin L, Li H, Zhao M, Zhang J, Wu H, Su L, Fu YX, Wang FS: B and T lymphocyte attenuator down-regulation by HIV-1 depends on type I interferon and contributes to T-cell hyperactivation. J Infect Dis. 2011 Jun 1;203(11):1668-78.
PubMed
Šedý JR, Balmert MO, Ware BC, Smith W, Němčovičová I, Norris PS, Miller BR, Aivazian D, Ware CF: A herpesvirus entry mediator mutein with selective agonist action for the inhibitory receptor B and T lymphocyte attenuator. J Biol Chem. 2017 Dec 22;292(51):21060-21070.
PubMed
Piancone F , Saresella M, Marventano I , La Rosa F , Zoppis M , Agostini S , Longhi R , Caputo D , Mendozzi L , Rovaris M , Clerici M: B lymphocytes in multiple sclerosis: Bregs and BTLA/CD272 expressing-CD19+ lymphocytes modulate disease severity. Sci Rep. 2016 Jul 14;6:29699. doi: 10.1038/srep29699.
PubMed
Cairo C, Surendran N, Harris KM, Mazan-Mamczarz K, Sakoda Y, Diaz-Mendez F, Tamada K, Gartenhaus RB, Mann DL, Pauza CD: Vγ2Vδ2 T cell costimulation increases NK cell killing of monocyte-derived dendritic cells. Immunology. 2014 Sep 16;144(3):422-430.
PubMed
Variant
0.1 mg
11-877-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD