Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD272 PE

Anti-Hu CD272 PE

Regulatory status

RUO

Antigen

CD272

Clone

MIH26

Format

Reactivity

Human, Non-human primates

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-877-T100

Ask for delivery term HERE

248.60 USD

Variant

100 tests

1P-877-T100

Ask for delivery term HERE

248.60 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a kappa

Specificity

The mouse monoclonal antibody MIH26 recognizes an extracellular epitope of CD272, a transmembrane glycoprotein serving as a negative regulator of the activation in various leukocyte types.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human, Non-human primates

Immunogen

cells transfected with human CD272

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

BTLA1

Antigen description

CD272, a type I transmembrane glycoprotein, contains in its intracellular domain two ITIM sequences, which are upon CD272 triggering phosphorylated and recruit SHP phosphatases to attenuate cell activation. CD272 is expressed on B and T lymphocytes, NK cells, dendritic cells, and macrophages, and its ligand is CD270. Defects in CD272-CD270 inhibitory mechanism lead to autoimmune diseases. Overexpression of CD272 is a marker of tolerant T cells.

Entrez Gene ID 151888

UniProt ID Q7Z6A9

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD272 (MIH26) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD272 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD272 (MIH26) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Ramsay AG , Clear AJ, Fatah R, Gribben JG: Multiple inhibitory ligands induce impaired T-cell immunologic synapse function in chronic lymphocytic leukemia that can be blocked with lenalidomide: establishing a reversible immune evasion mechanism in human cancer. Blood. 2012 Aug 16;120(7):1412-21.
PubMed

Zhang Z, Xu X, Lu J, Zhang S, Gu L, Fu J, Jin L, Li H, Zhao M, Zhang J, Wu H, Su L, Fu YX, Wang FS: B and T lymphocyte attenuator down-regulation by HIV-1 depends on type I interferon and contributes to T-cell hyperactivation. J Infect Dis. 2011 Jun 1;203(11):1668-78.
PubMed

Šedý JR, Balmert MO, Ware BC, Smith W, Němčovičová I, Norris PS, Miller BR, Aivazian D, Ware CF: A herpesvirus entry mediator mutein with selective agonist action for the inhibitory receptor B and T lymphocyte attenuator. J Biol Chem. 2017 Dec 22;292(51):21060-21070.
PubMed

Piancone F , Saresella M, Marventano I , La Rosa F , Zoppis M , Agostini S , Longhi R , Caputo D , Mendozzi L , Rovaris M , Clerici M: B lymphocytes in multiple sclerosis: Bregs and BTLA/CD272 expressing-CD19+ lymphocytes modulate disease severity. Sci Rep. 2016 Jul 14;6:29699. doi: 10.1038/srep29699.
PubMed

Cairo C, Surendran N, Harris KM, Mazan-Mamczarz K, Sakoda Y, Diaz-Mendez F, Tamada K, Gartenhaus RB, Mann DL, Pauza CD: Vγ2Vδ2 T cell costimulation increases NK cell killing of monocyte-derived dendritic cells. Immunology. 2014 Sep 16;144(3):422-430.
PubMed