Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD271 Purified

Anti-Hu CD271 Purified

Regulatory status

RUO

Antigen

CD271

Clone

NGFR5

Format

Purified

Reactivity

Human, Rabbit, Cat, Non-human primates, Ferret

Application

IP, MC-CyTOF, WB, FC (QC tested), IHC-P, ICC

Variant

0.1 mg

11-642-C100

In stock

147.40 USD

0.025 mg

11-642-C025

Delivery 1 week

73.70 USD

Variant

0.1 mg

11-642-C100

In stock

147.40 USD

0.025 mg

11-642-C025

Delivery 1 week

73.70 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody NGFR5 (originally C34C) recognizes an epitope within ammino acids 1 - 160 of CD271/NGFR, a 75 kDa transmembrane glycoprotein of the TNFR superfamily.

Workshop

HCDM listed

Application

IP, MC-CyTOF, WB, FC (QC tested), IHC-P, ICC

Application details

Immunohistochemistry (paraffin sections): Recommended dilution: 1-2 μg/ml; Positive tissue: melanoma, heat-mediated antigen retrieval.
Flow cytometry: Extracellular and intracellular staining; recommended dilution: 1-4 µg/ml.

Reactivity

Human, Rabbit, Cat, Non-human primates, Ferret

Negative species

Mouse, Rat

Immunogen

Purified CD271 protein isolated from human melanoma cell line A875

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

TNFRSF16, NGFR, p75NTR, p75NGFR

Antigen description

CD271 / NGFR, also known as p75NGFR or p75NTR, is a 75 kDa low affinity receptor for the NGF (nerve growth factor), BDNF (brain-derived growth factor), and other neurotrophins, such as NT3 and NT4/5. Unlike other members of the tumor necrosis factor receptor superfamily of transmembrane proteins, CD271 has unique intracellular domain structure (lacks catalytic activity) and downstream signaling partners. Triggered by its ligands CD271 affects growth, differentiation, migration and death of the nervous system cells.

Entrez Gene ID 4804

UniProt ID P08138

Separation of SK-MEL-30 cells stained using anti-CD271 (NGFR5) purified antibody (concentration in sample 1,7 μg/ml, GAM APC, red-filled) from SK-MEL-30 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

Immunohistochemistry staining of human testis (paraffin sections) using anti-CD271 (NGFR5). Commercially tested by LifeSpan BioSciences.

Mass cytometry (surface staining) of brain tumor cells with anti-CD271 (NGFR5) 143Nd. Gated on singlets.

Product specific references:

Thompson SJ, Schatteman GC, Gown AM, Bothwell M: A monoclonal antibody against nerve growth factor receptor. Immunohistochemical analysis of normal and neoplastic human tissue. Am J Clin Pathol. 1989 Oct;92(4):415-23.
PubMed

Marano N, Dietzschold B, Earley JJ Jr, Schatteman G, Thompson S, Grob P, Ross AH, Bothwell M, Atkinson BF, Koprowski H: Purification and amino terminal sequencing of human melanoma nerve growth factor receptor. J Neurochem. 1987 Jan;48(1):225-32.
PubMed

Schatteman GC, Gibbs L, Lanahan AA, Claude P, Bothwell M: Expression of NGF receptor in the developing and adult primate central nervous system. J Neurosci. 1988 Mar;8(3):860-73.
PubMed

Alpers CE, Hudkins KL, Ferguson M, Johnson RJ, Schatteman GC, Bothwell M: Nerve growth factor receptor expression in fetal, mature, and diseased human kidneys. Lab Invest. 1993 Dec;69(6):703-13.
PubMed