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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Hu CD271 Purified
Anti-Hu CD271 Purified
Regulatory status
RUO
Antigen
CD271
Clone
NGFR5
Format
Purified
Reactivity
Human, Rabbit, Cat, Non-human primates, Ferret
Application
IP, MC (CyTOF), WB, FC (QC tested), IHC(P), ICC
Variant
0.1 mg
11-642-C100
In stock
130.00 USD
0.025 mg
11-642-C025
Delivery 1 week
65.00 USD
Variant
0.1 mg
11-642-C100
In stock
130.00 USD
0.025 mg
11-642-C025
Delivery 1 week
65.00 USD
Contact distributor
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody NGFR5 (originally C34C) recognizes an epitope within ammino acids 1 - 160 of CD271/NGFR, a 75 kDa transmembrane glycoprotein of the TNFR superfamily.
Workshop
HCDM listed
Application
IP, MC (CyTOF), WB, FC (QC tested), IHC(P), ICC
Application details
Immunohistochemistry (paraffin sections): Recommended dilution: 1-2 μg/ml; Positive tissue: melanoma, heat-mediated antigen retrieval.
Flow cytometry: Extracellular and intracellular staining; recommended dilution: 1-4 µg/ml.
Reactivity
Human, Rabbit, Cat, Non-human primates, Ferret
Negative species
Mouse, Rat
Immunogen
Purified CD271 protein isolated from human melanoma cell line A875
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
TNFRSF16, NGFR, p75NTR, p75NGFR
Antigen description
CD271 / NGFR, also known as p75NGFR or p75NTR, is a 75 kDa low affinity receptor for the NGF (nerve growth factor), BDNF (brain-derived growth factor), and other neurotrophins, such as NT3 and NT4/5. Unlike other members of the tumor necrosis factor receptor superfamily of transmembrane proteins, CD271 has unique intracellular domain structure (lacks catalytic activity) and downstream signaling partners. Triggered by its ligands CD271 affects growth, differentiation, migration and death of the nervous system cells.
Entrez Gene ID
4804
UniProt ID
P08138
Separation of SK-MEL-30 cells stained using anti-CD271 (NGFR5) purified antibody (concentration in sample 1,7 μg/ml, GAM APC, red-filled) from SK-MEL-30 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).
Immunohistochemistry staining of human testis (paraffin sections) using anti-CD271 (NGFR5). Commercially tested by LifeSpan BioSciences.
Mass cytometry (surface staining) of brain tumor cells with anti-CD271 (NGFR5) 143Nd. Gated on singlets.
Product specific references:
Thompson SJ, Schatteman GC, Gown AM, Bothwell M: A monoclonal antibody against nerve growth factor receptor. Immunohistochemical analysis of normal and neoplastic human tissue. Am J Clin Pathol. 1989 Oct;92(4):415-23.
PubMed
Marano N, Dietzschold B, Earley JJ Jr, Schatteman G, Thompson S, Grob P, Ross AH, Bothwell M, Atkinson BF, Koprowski H: Purification and amino terminal sequencing of human melanoma nerve growth factor receptor. J Neurochem. 1987 Jan;48(1):225-32.
PubMed
Schatteman GC, Gibbs L, Lanahan AA, Claude P, Bothwell M: Expression of NGF receptor in the developing and adult primate central nervous system. J Neurosci. 1988 Mar;8(3):860-73.
PubMed
Schatteman GC, Gibbs L, Lanahan AA, Claude P, Bothwell M: Expression of NGF receptor in the developing and adult primate central nervous system. J Neurosci. 1988 Mar;8(3):860-73.
PubMed
Alpers CE, Hudkins KL, Ferguson M, Johnson RJ, Schatteman GC, Bothwell M: Nerve growth factor receptor expression in fetal, mature, and diseased human kidneys. Lab Invest. 1993 Dec;69(6):703-13.
PubMed
Variant
0.1 mg
11-642-C100
In stock
130.00 USD
0.025 mg
11-642-C025
Delivery 1 week
65.00 USD
Variant
0.1 mg
11-642-C100
In stock
130.00 USD
0.025 mg
11-642-C025
Delivery 1 week
65.00 USD
Contact distributor
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SDS download
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