Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD270 Alexa Fluor<sup>®</sup> 488

Anti-Hu CD270 Alexa Fluor^® 488

Regulatory status

RUO

Antigen

CD270

Clone

CW10

Format

Alexa Fluor^® 488

Reactivity

Human

Application

FC (QC tested), ICC

Excitation laser

blue (488 nm)

Variant

100 tests

A4-911-T100

In stock

242.00 USD

Variant

100 tests

A4-911-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody CW10 recognizes an extracellular epitope on CD270, a type I transmembrane protein expressed on resting T cells, monocytes, and immature dendritic cells.

Application

FC (QC tested), ICC

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

recombinant human CD270

Preparation

Purified antibody is conjugated with Alexa Fluor^® 488 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Alexa Fluor^®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Other names

TNFRSF14, TR2, ATAR, HVEA, HVEM, LIGHTR

Antigen description

CD270 is a type I transmembrane protein of the TNFR superfamily, which is expressed on resting T cells, monocytes, and immature dendritic cells. Its ligands, CD258 and CD272, differ in effect on CD270 signaling. Whereas binding to CD258 provides a costimulatory signal, binding to CD272 gives to the cell an inhibitory signal. CD270 also is recognized by herpes simplex glycoprotein D. CD258-CD270 interaction and signaling is implicated in macrophage-derived foam cell-mediated development of atherosclerotic lesions.

Entrez Gene ID 8764

UniProt ID Q92956

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD270 (CW10) Alexa Fluor® 488 antibody (concentration in sample 0.56 μg/ml).

Anti-Hu CD270 Alexa Fluor® 488 antibody (clone CW10) specificity verification by flow cytometry

Anti-Hu CD270 Alexa Fluor® 488 antibody (concentration in sample 0.56 μg/ml, red-filled histogram) binds specifically to CD270 on surface of SK-MEL-30 cells (upper panel) and also main leukocyte subpopulations - neutrophil granulocytes, monocytes, lymphocytes (middle panels), but not to surface of blood debris including thrombocytes (lower panel). Level of non-specific binding was assessed using Mouse IgG1 isotype control Alexa Fluor® 488 antibody (MOPC-21) under same conditions (concentration in sample 0.56 μg/ml, black-dashed histogram).

Product specific references:

Whitbeck JC, Connolly SA, Willis SH, Hou W, Krummenacher C, de Leon MP, Lou H, Baribaud I, Eisenberg RJ, Cohen GH: Localization of the gD-binding region of the human herpes simplex virus receptor, HveA. J Virol. 2001 Jan;75(1):171-80.
PubMed

Stiles KM, Whitbeck JC, Lou H, Cohen GH, Eisenberg RJ, Krummenacher C: Herpes simplex virus glycoprotein D interferes with binding of herpesvirus entry mediator to its ligands through downregulation and direct competition. J Virol. 2010 Nov;84(22):11646-60.
PubMed