Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD27 Purified

Anti-Hu CD27 Purified

Regulatory status

RUO

Antigen

CD27

Clone

LT27

Format

Purified

Reactivity

Human

Application

FC (QC tested)

Variant

0.1 mg

11-308-C100

In stock

143.00 USD

0.025 mg

11-308-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-308-C100

In stock

143.00 USD

0.025 mg

11-308-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The antibody LT27 reacts with an extracellular epitope of CD27 (T14), a 50-55 kDa type I transmembrane glycoprotein (member of the TNF-receptor superfamily) expressed on medullary thymocytes, peripheral T lymphocytes, some B lymphocytes and NK cells.

Workshop

HLDA V: WS Code T T-CD27.01

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 2 μg/ml.

Reactivity

Human

Immunogen

Human peripheral blood lymphocytes

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

T14, TNFRSF7, LPFS2, S152, Tp55

Antigen description

CD27 is a transmembrane 55 kDa protein of the nerve growth factor-receptor family, expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. This sCD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70.

Entrez Gene ID 939

UniProt ID P26842

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD27 (LT27) purified antibody (concentration in sample 2 μg/ml, GAM APC).

Separation of human CD27 positive lymphocytes (red-filled) from neutrofil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD27 (LT27) purified antibody (concentration in sample 2 μg/ml, GAM APC).

General references:

Yun LW, Decarlo AA, Hunter N: Blockade of protease-activated receptors on T cells correlates with altered proteolysis of CD27 by gingipains of Porphyromonas gingivalis. Clin Exp Immunol. 2007 Nov;150(2):217-29.
PubMed

Kara IO, Sahin B, Gunesacar R: Expression of soluble CD27 and interleukins-8 and -10 in B-cell chronic lymphocytic leukemia: correlation with disease stage and prognosis. Adv Ther. 2007 Jan-Feb;24(1):29-40.
PubMed

Molica S, Vitelli G, Levato D, Crispino G, Dell'Olio M, Dattilo A, Matera R, Gandolfo GM, Musto P. CD27 in B-cell chronic lymphocytic leukemia. Cellular expression, serum release and correlation with other soluble molecules belonging to nerve growth factor receptors (NGFr) superfamily. Haematologica. 1998 May;83(5):398-402.
PubMed

van Oers MH, Pals ST, Evers LM, van der Schoot CE, Koopman G, Bonfrer JM, Hintzen RQ, von dem Borne AE, van Lier RA: Expression and release of CD27 in human B-cell malignancies. Blood. 1993 Dec 1;82(11):3430-6.
PubMed

Product specific references:

Kanderova V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, Hrusak O, Lund-Johansen F, Kalina T: High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016 Apr;15(4):1246-61.
PubMed