Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD268 Purified

Anti-Hu CD268 Purified

Regulatory status

RUO

Antigen

CD268

Clone

11C1

Format

Purified

Reactivity

Human

Application

FC (QC tested), IHC(F), IHC(P)

Variant

0.1 mg

11-163-C100

In stock

170.00 USD

0.025 mg

11-163-C025

Delivery 1 week

85.00 USD

Variant

0.1 mg

11-163-C100

In stock

170.00 USD

0.025 mg

11-163-C025

Delivery 1 week

85.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody 11C1 recognizes an extracellular epitope of CD268 / BAFF R (B cell-activating factor receptor), a 19 kDa type III transmembrane protein expressed on resting B cells and CD4-positive T cells, but down regulated after activation.

Workshop

HLDA VIII

Application

FC (QC tested), IHC(F), IHC(P)

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Human

Immunogen

human CD268-transfected murine L1.2 cells

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

TNFRSF13C, BAFFR, CVID4, BAFF-R, BROMIX, prolixin

Antigen description

CD268 / BAFF R is a TNFR family receptor that binds the B-cell-activating factor (CD257 / BAFF). Splice variants of CD268 have been observed both in man and mouse. A naturally occurring mutation of CD268 in A/WySnJ mice is associated with low number of mature B cells, but with normal B cell precursors. The role of BAFF in B-cell survival and activation make CD268 a potential diagnostic reagent. It may be involved in survival of B-cell malignancies. Experimental administration of a CD268-Fc fusion protein suppresses antibody responses. In T cells the CD268 costimulates their activation and proliferation. Defects in CD268 cause the common variable immunodeficiency 4 (CVID4).

Entrez Gene ID 115650

UniProt ID Q96RJ3

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD268 (11C1) purified antibody (concentration in sample 0,6 μg/ml, GAM APC).

Separation of human CD268 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD268 (11C1) purified antibody (concentration in sample 0,6 μg/ml, GAM APC).

Product specific references:

Schneppenheim J, Hüttl S, Kruchen A, Fluhrer R, Müller I, Saftig P, Schneppenheim R, Martin CL, Schröder B: Signal-peptide-peptidase-like 2a is required for CD74 intramembrane proteolysis in human B cells. Biochem Biophys Res Commun. 2014 Aug 15;451(1):48-53.
PubMed

Yoshimoto K, Tanaka M, Kojima M, Setoyama Y, Kameda H, Suzuki K, Tsuzaka K, Ogawa Y, Tsubota K, Abe T, Takeuchi T: Regulatory mechanisms for the production of BAFF and IL-6 are impaired in monocytes of patients of primary Sjögren's syndrome. Arthritis Res Ther. 2011;13(5):R170.
PubMed

Proust A, Rincé P, Creidy R, Lazure T, Joab I, Garçon L, Fabre M, Guettier C, Raphael M: p52 Activation in monomorphic B-cell posttransplant lymphoproliferative disorder/diffuse large B-cell lymphoma without BAFF-R expression. Am J Pathol. 2011 Oct;179(4):1630-7.
PubMed