Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD267 PE

Anti-Hu CD267 PE

Regulatory status

RUO

Antigen

CD267

Clone

1A1

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-162-T100

In stock

248.60 USD

25 tests

1P-162-T025

Delivery 1 week

124.30 USD

Variant

100 tests

1P-162-T100

In stock

248.60 USD

25 tests

1P-162-T025

Delivery 1 week

124.30 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Rat IgG2a kappa

Specificity

The rat monoclonal antibody 1A1 recognizes an extracellular epitope of CD267 / TACI, a 32 kDa type III transmembrane protein expressed by B cells and possibly by some activated T cells.

Workshop

HLDA VIII

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

CD267-transfected RBL cells

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

RYZN, TACI, CVID2, IGAD2, TNFRSF14B

Antigen description

CD267 / TACI (transmembrane activator calcium modulator and cyclophilin ligand interactor), a TNFR superfamily transmembrane protein, is expressed on B cells (predominantly on CD27+ memory cells), multiple myeloma cells and B cell chronic lymphocytic leukemia (B-CLL). Its triggering leads to activation of the transcription factors NFAT, AP1, and NF-kappa-B. It plays a crucial role in humoral immunity. Mutations in CD267 are associated with common variable immunodeficiency and IgA deficiency.

Entrez Gene ID 23495

UniProt ID O14836

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD267 (1A1) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD267 (1A1) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD19 (LT19) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD267 positive B cells (red-filled) from CD267 negative CD19 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD267 (1A1) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Koarada S, Tada Y, Suematsu R, Soejima S, Inoue H, Ohta A, Nagasawa K: Phenotyping of P105-negative B cell subsets in patients with systemic lupus erythematosus. Clin Dev Immunol. 2012;2012:198206.
PubMed