Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD261 Purified Low Endotoxin

Anti-Hu CD261 Purified Low Endotoxin

Anti-Hu CD261 Purified Low Endotoxin
Regulatory status
RUO
Antigen
CD261
Clone
DR-4-02
Format
Purified Low Endotoxin
Reactivity
Human
Application
IP, FC (QC tested), ICC, FUNC
Variant
0.1 mg
12-403-C100
In stock
180.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody DR-4-02 recognizes an extracellular epitope of TRAIL-R1 (DR4), a human death receptor 4 expressed in most human tissues (spleen, peripheral blood leucocytes, thymus) and in a variety of tumour-derived cell lines.
Workshop
HLDA X
Application
IP, FC (QC tested), ICC, FUNC
Application details
Functional application: Soluble antibody DR-4-02 blocks apoptosis triggered by a ligand (TRAIL). Plastic-immobilized (cross-linked) DR-4-02 antibody induces apoptosis in sensitive cells. Recommended dilution of antibody: 2-3 μg/ml in cultivation medium, final concentration of TRAIL: 20-200 ng/ml; it is recommended to add the antibody 15 min before addition of TRAIL.
Flow cytometry: Recommended dilution: 2-4 μg/ml.
Reactivity
Human
Immunogen
Fusion protein containing the extracellular part of TRAIL-R1 and the constant part of the heavy chain of the human IgG1.
Other names
DR4, APO2, TNFRSF10A, TRAILR1, TRAIL-R1
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Low endotoxin azide free phosphate buffered saline (PBS) solution, 0.2 μm filter sterilized. Endotoxin level is less than 0.01 EU/μg of the protein, as determined by the LAL test.
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
12-403 FC
Flow cytometry analysis of TRAIL-R1 expression on the surface of hematopoietic cell lines. Cells were stained with purified anti-TRAIL-R1 purified antibodies (low endotoxin) followed by Goat anti-mouse IgG PE.

General references:

Corallini F, Milani D, Nicolin V, Secchiero P: TRAIL, caspases and maturation of normal and leukemic myeloid precursors. Leuk Lymphoma. 2006 Aug;47(8):1459-68.
PubMed

Product specific references:

Simova S, Klima M, Cermak L, Sourkova V, Andera L: Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane. Apoptosis. 2008 Mar;13(3):423-36.
PubMed
Variant
0.1 mg
12-403-C100
In stock
180.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD
Contact distributor
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SDS download
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