Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD261 Purified Low Endotoxin

Anti-Hu CD261 Purified Low Endotoxin

Regulatory status

RUO

Antigen

CD261

Clone

DR-4-02

Format

Purified Low Endotoxin

Reactivity

Human

Application

IP, FC (QC tested), ICC, FUNC

Variant

0.1 mg

12-403-C100

In stock

180.00 USD

Variant

0.1 mg

11-261-C100

In stock

180.00 USD

Contact distributor

Product details

Description

Images

References

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody DR-4-02 recognizes an extracellular epitope of TRAIL-R1 (DR4), a human death receptor 4 expressed in most human tissues (spleen, peripheral blood leucocytes, thymus) and in a variety of tumour-derived cell lines.

Workshop

HLDA X

Application

IP, FC (QC tested), ICC, FUNC

Application details

Functional application: Soluble antibody DR-4-02 blocks apoptosis triggered by a ligand (TRAIL). Plastic-immobilized (cross-linked) DR-4-02 antibody induces apoptosis in sensitive cells. Recommended dilution of antibody: 2-3 μg/ml in cultivation medium, final concentration of TRAIL: 20-200 ng/ml; it is recommended to add the antibody 15 min before addition of TRAIL.
Flow cytometry: Recommended dilution: 2-4 μg/ml.

Reactivity

Human

Immunogen

Fusion protein containing the extracellular part of TRAIL-R1 and the constant part of the heavy chain of the human IgG1.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

DR4, APO2, TNFRSF10A, TRAILR1, TRAIL-R1

Antigen description

TRAIL-R1 (CD261, DR4) is a type I transmembrane protein, also called TRAIL receptor 1. The ligand for this DR4 death receptor has been identified and termed TRAIL, which is a member of the TNF family. DR4, as many other receptors (Fas, TNFR1, etc.), mediates apoptosis and NF kappaB activation in many cells and tissues. Apoptosis, a programmed cell death, is a operating process in normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by coupled of certain cytokines (TNF family - TNF, Fas ligand) and their death domain containing receptors (TNFR1, Fas receptor).

Entrez Gene ID 8797

UniProt ID O00220

Flow cytometry analysis of TRAIL-R1 expression on the surface of hematopoietic cell lines. Cells were stained with purified anti-TRAIL-R1 purified antibodies (low endotoxin) followed by Goat anti-mouse IgG PE.

General references:

Corallini F, Milani D, Nicolin V, Secchiero P: TRAIL, caspases and maturation of normal and leukemic myeloid precursors. Leuk Lymphoma. 2006 Aug;47(8):1459-68.
PubMed

Product specific references:

Simova S, Klima M, Cermak L, Sourkova V, Andera L: Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane. Apoptosis. 2008 Mar;13(3):423-36.
PubMed

Variant

0.1 mg

12-403-C100

In stock

180.00 USD

Variant

0.1 mg

11-261-C100

In stock

180.00 USD

Contact distributor

Datasheet download