Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD253 Purified Low Endotoxin

Anti-Hu CD253 Purified Low Endotoxin

Regulatory status

RUO

Antigen

CD253

Clone

2E5

Format

Purified Low Endotoxin

Reactivity

Human

Application

FC (QC tested), FUNC

Variant

0.1 mg

12-316-C100

In stock

180.00 USD

Variant

0.1 mg

12-316-C100

In stock

180.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody 2E5 reacts with an extracellular epitope within C-terminal half of TRAIL (APO-2L), a 21 kDa cytotoxic protein, activator of rapid apoptosis in tumor cells. TRAIL is mainly expressed in spleen, lung, prostate and also in many other tissues.

Workshop

HLDA IX

Application

FC (QC tested), FUNC

Application details

Functional application: The antibody 2E5 has high neutralizing activity for human TRAIL in biological assays.
Flow cytometry: Recommended dilution: 1-10 μg/ml.

Reactivity

Human

Negative species

Mouse

Immunogen

Recombinant soluble fragment (aa 95-281) of human TRAIL.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

TRAIL, Apo-2 ligand, Apo-2L, TNFSF10, APO2L, TNLG6A

Antigen description

Human CD253 / TRAIL (TNF-related apoptosis inducing ligand), also called Apo2, is a type II membrane protein from the TNF family. TRAIL is a cytotoxic protein which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL-induced apotosis, is achieved through binding to two dealth-signaling receptors, DR4 (CD261 / TRAIL-R1) and DR5 (CD262 / TRAIL-R2).

Entrez Gene ID 8743

UniProt ID P50591

Functional applicvation example: Apoptosis induced in JURKAT human T cell leukemia cell line by soluble recombinant human TRAIL is completely blocked by anti-human TRAIL (2E5). The neutralizing activity of the antibody 2E5 has been confirmed with various sources of soluble recombinant human TRAIL. A - medium B - recombinant TRAIL C - recombinant TRAIL + anti-human TRAIL (2E5; 0.06 μg/ml) D - recombinant TRAIL + anti-human TRAIL (2E5; 0.24 μg/ml) E - recombinant TRAIL + Isotype mouse IgG1 control

Product specific references:

Hyer ML, Croxton R, Krajewska M, Krajewski S, Kress CL, Lu M, Suh N, Sporn MB, Cryns VL, Zapata JM, Reed JC: Synthetic triterpenoids cooperate with tumor necrosis factor-related apoptosis-inducing ligand to induce apoptosis of breast cancer cells. Cancer Res. 2005 Jun 1;65(11):4799-808.
PubMed

Plasilova M, Zivny J, Jelinek J, Neuwirtova R, Cermak J, Necas E, Andera L, Stopka T: TRAIL (Apo2L) suppresses growth of primary human leukemia and myelodysplasia progenitors. Leukemia. 2002 Jan;16(1):67-73.
PubMed