Anti-Hu CD253 PE

Anti-Hu CD253 PE
Regulatory status
RUO
Antigen
CD253
Clone
2E5
Format
PE
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
0.1 mg
1P-316-C100
In stock
242.00 USD

0.025 mg
1P-316-C025
Delivery 1 week
121.00 USD
Variant
0.1 mg
1P-316-C100
In stock
242.00 USD

0.025 mg
1P-316-C025
Delivery 1 week
121.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The antibody 2E5 reacts with an extracellular epitope within C-terminal half of TRAIL (APO-2L), a 21 kDa cytotoxic protein, activator of rapid apoptosis in tumor cells. TRAIL is mainly expressed in spleen, lung, prostate and also in many other tissues.
Workshop
HLDA IX
Application
Application details
Flow cytometry: Recommended dilution: 1-5 μg/ml.
Reactivity
Human
Negative species
Mouse
Immunogen
Recombinant soluble fragment (aa 95-281) of human TRAIL.
Concentration
0.1 mg/ml
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
TRAIL, Apo-2 ligand, Apo-2L, TNFSF10, APO2L, TNLG6A
Antigen description
Human CD253 / TRAIL (TNF-related apoptosis inducing ligand), also called Apo2, is a type II membrane protein from the TNF family. TRAIL is a cytotoxic protein which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL-induced apotosis, is achieved through binding to two dealth-signaling receptors, DR4 (CD261 / TRAIL-R1) and DR5 (CD262 / TRAIL-R2).
Entrez Gene ID 8743
UniProt ID P50591
1P-316_FC_histogram
Anti-Hu CD253 PE antibody (clone 2E5) specificity verification by flow cytometry

Colorectal cancer cell line HCT-116 with the eliminated expression of pro-apoptotic proteins BAK and BAX (prepared using CRISPR Cas9 gene editing approach) was used as host for CD253 (TRAIL) transfection. BAK/BAX double knockout cells are resistant to TRAIL-induced apoptosis.
Anti-Hu CD253 PE (concentration in sample 5 μg/ml, red-filled histogram) binds specifically to surface CD253/TRAIL in transfected cells (upper panel), but not to the parent HCT-166 BAK/BAK double KO (lower panel). Level of non-specific binding was assessed using Mouse IgG1 isotype control PE (MOPC-21) under same conditions (concentration in sample 5 μg/ml, black-dashed histogram).

Product specific references:

Hyer ML, Croxton R, Krajewska M, Krajewski S, Kress CL, Lu M, Suh N, Sporn MB, Cryns VL, Zapata JM, Reed JC: Synthetic triterpenoids cooperate with tumor necrosis factor-related apoptosis-inducing ligand to induce apoptosis of breast cancer cells. Cancer Res. 2005 Jun 1;65(11):4799-808.
PubMed
Plasilova M, Zivny J, Jelinek J, Neuwirtova R, Cermak J, Necas E, Andera L, Stopka T: TRAIL (Apo2L) suppresses growth of primary human leukemia and myelodysplasia progenitors. Leukemia. 2002 Jan;16(1):67-73.
PubMed
Variant
0.1 mg
1P-316-C100
In stock
242.00 USD

0.025 mg
1P-316-C025
Delivery 1 week
121.00 USD
Variant
0.1 mg
1P-316-C100
In stock
242.00 USD

0.025 mg
1P-316-C025
Delivery 1 week
121.00 USD

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