Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD253 PE

Anti-Hu CD253 PE

Regulatory status

RUO

Antigen

CD253

Clone

2E5

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

0.1 mg

1P-316-C100

In stock

242.00 USD

0.025 mg

1P-316-C025

Delivery 1 week

121.00 USD

Variant

0.1 mg

1P-316-C100

In stock

242.00 USD

0.025 mg

1P-316-C025

Delivery 1 week

121.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody 2E5 reacts with an extracellular epitope within C-terminal half of TRAIL (APO-2L), a 21 kDa cytotoxic protein, activator of rapid apoptosis in tumor cells. TRAIL is mainly expressed in spleen, lung, prostate and also in many other tissues.

Workshop

HLDA IX

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-5 μg/ml.

Reactivity

Human

Negative species

Mouse

Immunogen

Recombinant soluble fragment (aa 95-281) of human TRAIL.

Concentration

0.1 mg/ml

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

TRAIL, Apo-2 ligand, Apo-2L, TNFSF10, APO2L, TNLG6A

Antigen description

Human CD253 / TRAIL (TNF-related apoptosis inducing ligand), also called Apo2, is a type II membrane protein from the TNF family. TRAIL is a cytotoxic protein which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL-induced apotosis, is achieved through binding to two dealth-signaling receptors, DR4 (CD261 / TRAIL-R1) and DR5 (CD262 / TRAIL-R2).

Entrez Gene ID 8743

UniProt ID P50591

Anti-Hu CD253 PE antibody (clone 2E5) specificity verification by flow cytometry

Colorectal cancer cell line HCT-116 with the eliminated expression of pro-apoptotic proteins BAK and BAX (prepared using CRISPR Cas9 gene editing approach) was used as host for CD253 (TRAIL) transfection. BAK/BAX double knockout cells are resistant to TRAIL-induced apoptosis.
Anti-Hu CD253 PE (concentration in sample 5 μg/ml, red-filled histogram) binds specifically to surface CD253/TRAIL in transfected cells (upper panel), but not to the parent HCT-166 BAK/BAK double KO (lower panel). Level of non-specific binding was assessed using Mouse IgG1 isotype control PE (MOPC-21) under same conditions (concentration in sample 5 μg/ml, black-dashed histogram).

Product specific references:

Hyer ML, Croxton R, Krajewska M, Krajewski S, Kress CL, Lu M, Suh N, Sporn MB, Cryns VL, Zapata JM, Reed JC: Synthetic triterpenoids cooperate with tumor necrosis factor-related apoptosis-inducing ligand to induce apoptosis of breast cancer cells. Cancer Res. 2005 Jun 1;65(11):4799-808.
PubMed

Plasilova M, Zivny J, Jelinek J, Neuwirtova R, Cermak J, Necas E, Andera L, Stopka T: TRAIL (Apo2L) suppresses growth of primary human leukemia and myelodysplasia progenitors. Leukemia. 2002 Jan;16(1):67-73.
PubMed