Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD25 Purified

Anti-Hu CD25 Purified

Regulatory status

RUO

Antigen

CD25

Clone

MEM-140

Format

Purified

Reactivity

Human

Application

IP, FC (QC tested)

Variant

0.1 mg

11-299-C100

In stock

143.00 USD

0.025 mg

11-299-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-299-C100

In stock

143.00 USD

0.025 mg

11-299-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgM

Specificity

The antibody MEM-140 reacts with an extracellular epitope of CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4⁺ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.

Workshop

HLDA VI: WS Code C C-54

Application

IP, FC (QC tested)

Application details

Immunoprecipitation: Excellent for immunoisolation of CD25⁺ cells.
Flow cytometry: Recommended dilution: 1 μg/ml.

Reactivity

Human

Immunogen

PHA-activated peripheral blood leucocytes

Concentration

1 mg/ml

Preparation

Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Formulation

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

IL-2R α chain, IL-2-RA, IL2-RA, p55, TAC antigen, IDDM10, TCGFR

Antigen description

CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas.

Entrez Gene ID 3559

UniProt ID P01589

Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononclear cells stained using anti-human CD25 (MEM-140) purified antibody (concentration in sample 0,6 μg/ml, GAM APC, red) and human PHA stimulated peripheral blood mononclear cells unstained by primary antibody (GAM APC, blue).

General references:

Sotlar K, Horny HP, Simonitsch I, Krokowski M, Aichberger KJ, Mayerhofer M, Printz D, Fritsch G, Valent P: CD25 indicates the neoplastic phenotype of mast cells: a novel immunohistochemical marker for the diagnosis of systemic mastocytosis (SM) in routinely processed bone marrow biopsy specimens. Am J Surg Pathol. 2004 Oct;28(10):1319-25.
PubMed

Morris JC, Waldmann TA: Advances in interleukin 2 receptor targeted treatment. Ann Rheum Dis. 2000 Nov;59 Suppl 1:i109-14.
PubMed

Scheibenbogen C, Keilholz U, Richter M, Andreesen R, Hunstein W: The interleukin-2 receptor in human monocytes and macrophages: regulation of expression and release of the alpha and beta chains (p55 and p75). Res Immunol. 1992 Jan;143(1):33-7.
PubMed

Lai KN, Leung JC, Lai FM: Soluble interleukin 2 receptor release, interleukin 2 production, and interleukin 2 receptor expression in activated T-lymphocytes in vitro. Pathology. 1991 Jul;23(3):224-8.
PubMed