Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD245 PE

Anti-Hu CD245 PE

Regulatory status

RUO

Antigen

CD245

Clone

DY12

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-941-T100

In stock

242.00 USD

Variant

100 tests

1P-941-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody DY12 recognizes an extracellular epitope of CD245, a transmembrane protein expressed mainly by peripheral blood lymphocytes, NK cells, monocytes, granulocytes and platelets.

Workshop

HLDA VI, HLDA VII

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

human NK-type YT2C2 cells

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

p220/240, DY12, DY35

Antigen description

CD245 is a 220-250 kDa transmembrane protein of unknown structure. It is expressed at lower level on resting blood lymphocytes, granulocytes, platelets, and thymocytes, and at high level on monocytes and on IL-2-dependent T cell lines. It is also expressed on Ewing sarcoma and osteosarcoma cell lines. CD245 seems to act as a costimulation molecule of T and NK cells.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD245 (DY12) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD245 (DY12) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).