Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD244 FITC

Anti-Hu CD244 FITC

Regulatory status

RUO

Antigen

CD244

Clone

2-69

Format

FITC

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1F-870-T100

In stock

198.00 USD

Variant

100 tests

1F-870-T100

In stock

198.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a kappa

Specificity

The mouse monoclonal antibody 2-69 recognizes an extracellular epitope of CD244, a transmembrane glycoprotein expressed on NK cells, basophils, monocytes, an some T cell subsets.

Workshop

HLDA VII

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

human NK cells

Preparation

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

SLAMF4, 2B4, NAIL, NMRK

Antigen description

CD244 is a 70 kDa type I transmembrane glycoprotein containing four ITSM domains in its cytoplasmic part, serving as docking sites for both activating and inhibitory signaling molecules. Its extracellular ligand is CD48. CD244 is involved in non-MHC restricted cytotoxic activity of NK cells and acts as a costimulatory ligand for both NK cells and T cells. Interaction of CD244 with CD48 on T cells leads to the activation of both, but interaction with CD48 on other cells (e.g. tumor cells) can lead to inhibition of NK cell functions.

Entrez Gene ID 51744

UniProt ID Q9BZW8

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD244 (2-69) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry multicolor surface staining pattern of human peripheral blood mononuclear cells using anti-human CD244 (2-69) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood) and anti-human CD123 (6H6) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD244 positive CD123 positive basophil granulocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD244 (2-69) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Tassi I, Colonna M: The cytotoxicity receptor CRACC (CS-1) recruits EAT-2 and activates the PI3K and phospholipase Cgamma signaling pathways in human NK cells. J Immunol. 2005 Dec 15;175(12):7996-8002.
PubMed

Liechti T, Roederer M: OMIP-058: 30-Parameter Flow Cytometry Panel to Characterize iNKT, NK, Unconventional and Conventional T Cells. Cytometry A. 2019 Sep;95(9):946-951.
PubMed

Thanapati S, Das R, Tripathy AS: Phenotypic and functional analyses of NK and NKT-like populations during the early stages of chikungunya infection. Front Microbiol. 2015 Sep 1;6:895.
PubMed

Lee HR, Son CH, Koh EK, Bae JH, Kang CD, Yang K, Park YS: Expansion of cytotoxic natural killer cells using irradiated autologous peripheral blood mononuclear cells and anti-CD16 antibody. Sci Rep. 2017 Sep 11;7(1):11075
PubMed