Anti-Hu CD244 APC

Anti-Hu CD244 APC
Regulatory status
RUO
Antigen
CD244
Clone
2-69
Format
APC
Reactivity
Human
Application
Excitation laser
red (633 nm)
Variant
100 tests
1A-870-T100
In stock
220.00 USD
Variant
100 tests
1A-870-T100
In stock
220.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG2a kappa
Specificity
The mouse monoclonal antibody 2-69 recognizes an extracellular epitope of CD244, a transmembrane glycoprotein expressed on NK cells, basophils, monocytes, an some T cell subsets.
Workshop
HLDA VII
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
human NK cells
Preparation
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
SLAMF4, 2B4, NAIL, NMRK
Antigen description
CD244 is a 70 kDa type I transmembrane glycoprotein containing four ITSM domains in its cytoplasmic part, serving as docking sites for both activating and inhibitory signaling molecules. Its extracellular ligand is CD48. CD244 is involved in non-MHC restricted cytotoxic activity of NK cells and acts as a costimulatory ligand for both NK cells and T cells. Interaction of CD244 with CD48 on T cells leads to the activation of both, but interaction with CD48 on other cells (e.g. tumor cells) can lead to inhibition of NK cell functions.
Entrez Gene ID 51744
UniProt ID Q9BZW8
1A-870_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD244 (2-69) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
1A-870_FC_Dot-plot
Flow cytometry multicolor surface staining pattern of human peripheral blood mononuclear cells using anti-human CD123 (6H6) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD244 (2-69) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
1A-870_FC_Histogram
Separation of human CD123 positive CD244 positive basophils (red-filled) from CD244 negative CD123 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD244 (2-69) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Tassi I, Colonna M: The cytotoxicity receptor CRACC (CS-1) recruits EAT-2 and activates the PI3K and phospholipase Cgamma signaling pathways in human NK cells. J Immunol. 2005 Dec 15;175(12):7996-8002.
PubMed
Liechti T, Roederer M: OMIP-058: 30-Parameter Flow Cytometry Panel to Characterize iNKT, NK, Unconventional and Conventional T Cells. Cytometry A. 2019 Sep;95(9):946-951.
PubMed
Thanapati S, Das R, Tripathy AS: Phenotypic and functional analyses of NK and NKT-like populations during the early stages of chikungunya infection. Front Microbiol. 2015 Sep 1;6:895.
PubMed
Lee HR, Son CH, Koh EK, Bae JH, Kang CD, Yang K, Park YS: Expansion of cytotoxic natural killer cells using irradiated autologous peripheral blood mononuclear cells and anti-CD16 antibody. Sci Rep. 2017 Sep 11;7(1):11075
PubMed
Variant
100 tests
1A-870-T100
In stock
220.00 USD
Variant
100 tests
1A-870-T100
In stock
220.00 USD

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