Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD243 Purified Low Endotoxin

Anti-Hu CD243 Purified Low Endotoxin

Regulatory status

RUO

Antigen

CD243

Clone

UIC2

Format

Purified Low Endotoxin

Reactivity

Human

Application

IP, FC (QC tested), IHC(F), IHC(P), FUNC

Variant

0.1 mg

12-764-C100

In stock

130.00 USD

Variant

0.1 mg

12-764-C100

In stock

130.00 USD

Contact distributor

Product details

Description

References

Isotype

Mouse IgG2a kappa

Specificity

The mouse monoclonal antibody UIC2 recognizes an extracellular epitope on CD243 (MDR-1), an approximately 170 kDa ABC transporter expressed on hematopoietic stem cells, B, T, and NK cells, or on many multidrug resistant cancer cells. This antibody preferentially recognizes CD243 in the process of transporting substrate.

Workshop

HLDA VII

Application

IP, FC (QC tested), IHC(F), IHC(P), FUNC

Application details

Functional application: inhibition.
Immunohistochemistry (paraffin sections): Heat mediated antigen retrieval in citrate buffer pH 6.
Flow cytometry: Recommended dilution: 1-4 µg/ml

Reactivity

Human

Negative species

Mouse, Rat

Immunogen

NIH 3T3 cells transfected with human CD243 (MDR-1) cDNA

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

P-Glycoprotein, CLCS, MDR1, PGY1, ABC20, GP170, ABCB1, ABC transporter

Antigen description

CD243, also known as multidrug resistant protein 1 (MDR-1) or P-glycoprotein (Pgp) is an ATP binding cassette (ABC)-containing efflux transporter for xenobiotic lipophilic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. It is expressed in many tissues, including the brain, liver, pancreas, testes, kidney, and blood (B, T, NK cells, but not monocytes).

Entrez Gene ID 5243

UniProt ID P08183

Product specific references:

Kelley DJ, Pavelic ZP, Gapany M, Stambrook P, Pavelic L, Gapany S, Gluckman JL: Detection of P-glycoprotein in squamous cell carcinomas of the head and neck. Arch Otolaryngol Head Neck Surg. 1993 Apr;119(4):411-4.
PubMed

Collnot EM, Baldes C, Schaefer UF, Edgar KJ, Wempe MF, Lehr CM: Vitamin E TPGS P-glycoprotein inhibition mechanism: influence on conformational flexibility, intracellular ATP levels, and role of time and site of access. Mol Pharm. 2010 Jun 7;7(3):642-51.
PubMed

Goda K, Fenyvesi F, Bacsó Z, Nagy H, Márián T, Megyeri A, Krasznai Z, Juhász I, Vecsernyés M, Szabó G Jr: Complete inhibition of P-glycoprotein by simultaneous treatment with a distinct class of modulators and the UIC2 monoclonal antibody. J Pharmacol Exp Ther. 2007 Jan;320(1):81-8.
PubMed

Mechetner EB, Roninson IB: Efficient inhibition of P-glycoprotein-mediated multidrug resistance with a monoclonal antibody. Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):5824-8.
PubMed

Chaudhary PM, Mechetner EB, Roninson IB: Expression and activity of the multidrug resistance P-glycoprotein in human peripheral blood lymphocytes. Blood. 1992 Dec 1;80(11):2735-9.
PubMed

Variant

0.1 mg

12-764-C100

In stock

130.00 USD

Variant

0.1 mg

12-764-C100

In stock

130.00 USD

Contact distributor

Datasheet download