Anti-Hu CD243 APC

Anti-Hu CD243 APC
Regulatory status
RUO
Antigen
CD243
Clone
UIC2
Format
APC
Reactivity
Human
Application
Excitation laser
red (633 nm)
Variant
100 tests
1A-764-T100
In stock
242.00 USD
Variant
100 tests
1A-764-T100
In stock
242.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG2a kappa
Specificity
The mouse monoclonal antibody UIC2 recognizes an extracellular epitope on CD243 (MDR-1), an approximately 170 kDa ABC transporter expressed on hematopoietic stem cells, B, T, and NK cells, or on many multidrug resistant cancer cells. This antibody preferentially recognizes CD243 in the process of transporting substrate.
Workshop
HLDA VII
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Negative species
Mouse, Rat
Immunogen
NIH 3T3 cells transfected with human CD243 (MDR-1) cDNA
Preparation
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
P-Glycoprotein, CLCS, MDR1, PGY1, ABC20, GP170, ABCB1, ABC transporter
Antigen description
CD243, also known as multidrug resistant protein 1 (MDR-1) or P-glycoprotein (Pgp) is an ATP binding cassette (ABC)-containing efflux transporter for xenobiotic lipophilic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. It is expressed in many tissues, including the brain, liver, pancreas, testes, kidney, and blood (B, T, NK cells, but not monocytes).
Entrez Gene ID 5243
UniProt ID P08183
1A-764_FC_Profil Značení
Flow cytometry surface staining pattern of HEP-G2 cell suspension stained using anti-human CD243 (UIC2) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).
1A-764_FC_Histogram
Separation of HEP-G2 cells (red-filled) from human peripheral whole blood cells (black-dashed) in flow cytometry analysis (surface staining) stained using anti-human anti-human CD243 (UIC2) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).

Product specific references:

Kelley DJ, Pavelic ZP, Gapany M, Stambrook P, Pavelic L, Gapany S, Gluckman JL: Detection of P-glycoprotein in squamous cell carcinomas of the head and neck. Arch Otolaryngol Head Neck Surg. 1993 Apr;119(4):411-4.
PubMed
Collnot EM, Baldes C, Schaefer UF, Edgar KJ, Wempe MF, Lehr CM: Vitamin E TPGS P-glycoprotein inhibition mechanism: influence on conformational flexibility, intracellular ATP levels, and role of time and site of access. Mol Pharm. 2010 Jun 7;7(3):642-51.
PubMed
Goda K, Fenyvesi F, Bacsó Z, Nagy H, Márián T, Megyeri A, Krasznai Z, Juhász I, Vecsernyés M, Szabó G Jr: Complete inhibition of P-glycoprotein by simultaneous treatment with a distinct class of modulators and the UIC2 monoclonal antibody. J Pharmacol Exp Ther. 2007 Jan;320(1):81-8.
PubMed
Mechetner EB, Roninson IB: Efficient inhibition of P-glycoprotein-mediated multidrug resistance with a monoclonal antibody. Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):5824-8.
PubMed
Chaudhary PM, Mechetner EB, Roninson IB: Expression and activity of the multidrug resistance P-glycoprotein in human peripheral blood lymphocytes. Blood. 1992 Dec 1;80(11):2735-9.
PubMed
Variant
100 tests
1A-764-T100
In stock
242.00 USD
Variant
100 tests
1A-764-T100
In stock
242.00 USD

Related products

-134%
Reg. statusCE IVD
ApplicationFC
100 ml
220.00 USD
-134%
Reg. statusCE IVD
ApplicationFC
100 ml
66.00 USD
-134%
Reg. statusRUO
ApplicationFC
100 ml
55.00 USD
-134%
CloneMOPC-173
Reg. statusRUO
ApplicationFC, FC(IC)
0.1 mg
198.00 USD