Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD24 APC-Cy™7

Anti-Hu CD24 APC-Cy™7

Regulatory status

RUO

Antigen

CD24

Clone

SN3

Format

APC-Cy™7

Reactivity

Human

Application

FC (QC tested)

Excitation laser

red (633 nm)

Variant

100 tests

T4-503-T100

In stock

250.00 USD

25 tests

T4-503-T025

In stock

125.00 USD

Variant

0.1 mg

11-261-C100

In stock

250.00 USD

0.1 mg

11-261-C100

In stock

125.00 USD

Contact distributor

Product details

Description

Images

References

Isotype

Mouse IgG1

Specificity

The antibody SN3 reacts with CD24, a 35-45 kDa heavily glycosylated cell surface antigen. CD24 is expressed by granulocytes, B lymphocytes and by some activated T cells and T cell malignancies. It is not expressed on human thymocytes.

Workshop

HLDA IV: WS Code B 136; HLDA V: WS Code B CD24.7

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

Glycoproteins purified from human NALM-1 cell line.

Preparation

Purified antibody is conjugated with activated tandem dye of allophycocyanin-cyanine 7 (APC-Cy™7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Cy™ and CyDye™ are registered trademarks of Cytiva.

Other names

HSA, nectadorin, SCLC4

Antigen description

CD24, also known as heat-stable antigen (HSA) or nectadorin, is a small mucin-like GPI-anchored extracellular membrane glycoprotein expressed on several cell types, including B cells. When B cells are activated and induced to further maturation, however, CD24 begins to disappear. CD24 seems to act as a gate-keeper for lipid rafts, thereby regulating the activity of integrins and other proteins such as the chemokine receptor CXCR4; it is also a ligand for P-selectin. CD24 triggering induces apoptosis of B cell precursors but not in mature resting B cells, where it instead inhibits their ability to proliferate in response to activation. CD24 expression is associated with invasiveness and poorer prognosis of carcinomas and is a marker of exosomes secreted into urine and amniotic fluid.

Entrez Gene ID 100133941

UniProt ID P25063

Flow cytometry analysis (surface staining) of human peripheral blood cells (lymphocyte gate) with anti-human CD24 (SN3) APC-Cy7.

General references:

Runz S, Mierke CT, Joumaa S, Behrens J, Fabry B, Altevogt P: CD24 induces localization of beta1 integrin to lipid raft domains. Biochem Biophys Res Commun. 2008 Jan 4;365(1):35-41.
PubMed

Chou YY, Jeng YM, Lee TT, Hu FC, Kao HL, Lin WC, Lai PL, Hu RH, Yuan RH: Cytoplasmic CD24 expression is a novel prognostic factor in diffuse-type gastric adenocarcinoma. Ann Surg Oncol. 2007 Oct;14(10):2748-58.
PubMed

Keller S, Rupp C, Stoeck A, Runz S, Fogel M, Lugert S, Hager HD, Abdel-Bakky MS, Gutwein P, Altevogt P: CD24 is a marker of exosomes secreted into urine and amniotic fluid. Kidney Int. 2007 Nov;72(9):1095-102.
PubMed

Schabath H, Runz S, Joumaa S, Altevogt P: CD24 affects CXCR4 function in pre-B lymphocytes and breast carcinoma cells. J Cell Sci. 2006 Jan 15;119(Pt 2):314-25.
PubMed

Suzuki T, Kiyokawa N, Taguchi T, Sekino T, Katagiri YU, Fujimoto J: CD24 induces apoptosis in human B cells via the glycolipid-enriched membrane domains/rafts-mediated signaling system. J Immunol. 2001 May 1;166(9):5567-77.
PubMed

Product specific references:

Všianská P, Říhová L, Varmužová T, Suská R, Kryukov F, Mikulášová A, Kupská R, Penka M, Pour L, Adam Z, Hájek R: Analysis of B-cell subpopulations in monoclonal gammopathies. Clin Lymphoma Myeloma Leuk. 2015 Apr;15(4):e61-71.
PubMed

Maliar A, Servais C, Waks T, Chmielewski M, Lavy R, Altevogt P, Abken H, Eshhar Z: Redirected T cells that target pancreatic adenocarcinoma antigens eliminate tumors and metastases in mice. Gastroenterology. 2012 Nov;143(5):1375-1384
PubMed

Fukukawa T, Matsuzaki H, Haruta Y, Hara H, Seon BK: New monoclonal antibodies SN3, SN3a, and SN3b directed to sialic acid of glycoprotein on human non-T leukemia cells. Exp Hematol. 1986 Oct;14(9):850-5.
PubMed

Barcos M, Pollard C, Fukukawa T, Seon BK: Follicular mantle zone cell subpopulations detected by monoclonal antibody SN3. Hematol Oncol. 1986 Oct-Dec;4(4):251-9.
PubMed

Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).

Solvason N, Kearney JF. The human fetal omentum: a site of B cell generation. J Exp Med. 1992 Feb 1;175(2):397-404.
PubMed

Fischer GF, Majdic O, Gadd S, Knapp W. Signal transduction in lymphocytic and myeloid cells via CD24, a new member of phosphoinositol-anchored membrane molecules. J Immunol. 1990 Jan 15;144(2):638-41.
PubMed

Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).

Sutherland DR, Illingworth A, Marinov I, Ortiz F, Andreasen J, Payne D, Wallace PK, Keeney M: ICCS/ESCCA consensus guidelines to detect GPI-deficient cells in paroxysmal nocturnal hemoglobinuria (PNH) and related disorders part 2 - reagent selection and assay optimization for high-sensitivity testing. Cytometry B Clin Cytom. 2018 Jan;94(1):23-48.
PubMed

Variant

100 tests

T4-503-T100

In stock

250.00 USD

25 tests

T4-503-T025

In stock

125.00 USD

Variant

0.1 mg

11-261-C100