Anti-Hu CD235ab FITC

Anti-Hu CD235ab FITC
Regulatory status
RUO
Antigen
CD235ab
Clone
HIR2
Format
FITC
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1F-419-T100
In stock
198.00 USD

25 tests
1F-419-T025
In stock
99.00 USD
Variant
100 tests
1F-419-T100
In stock
198.00 USD

25 tests
1F-419-T025
In stock
99.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG2b
Specificity
The mouse monoclonal antibody HIR2 recognizes an epitope within the N-terminal (extracellular) portion of CD235a (glycophorin A) and CD235b (glycophorin B). Its antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells.
Workshop
HLDA VII: WS Code 70299
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Synthetic peptide (Human, N-terminal)
Preparation
Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Glycophorin A/B, GYPA/B, GPA/B, PAS-2/3, Sialoglycoprotein alpha/beta, GPSAT, GPErik, MSN blood group
Antigen description
CD235a (glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to CD235b (glycophorin B, GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, a Streptococcus adhesin.
1F-419_FC
Flow cytometry analysis (surface staining) of human peripheral blood with anti-CD235ab (HIR2) FITC.

Product specific references:

Marino JH, Tan C, Taylor AA, Bentley C, Van De Wiele CJ, Ranne R, Paliotta M, Broughan TA, Teague TK: Differential IL-7 responses in developing human thymocytes. Hum Immunol. 2010 Apr;71(4):329-33.
PubMed
Sponaas AM, Moharrami NN, Feyzi E, Standal T, Holth Rustad E, Waage A, Sundan A: PDL1 expression on plasma and dendritic cells in myeloma bone marrow suggests benefit of targeted anti PD1-PDL1 therapy. PLoS One. 2015 Oct 7;10(10):e0139867.
PubMed
Kyttälä A, Moraghebi R, Valensisi C, Kettunen J, Andrus C, Pasumarthy KK, Nakanishi M, Nishimura K, Ohtaka M, Weltner J, Van Handel B, Parkkonen O, Sinisalo J, Jalanko A, Hawkins RD, Woods NB, Otonkoski T, Trokovic R: Genetic variability overrides the impact of parental cell type and determines iPSC differentiation potential.
PubMed
Woo AJ, Kim J, Xu J, Huang H, Cantor AB: Role of ZBP-89 in human globin gene regulation and erythroid differentiation. Blood. 2011 Sep 29;118(13):3684-93.
PubMed
Variant
100 tests
1F-419-T100
In stock
198.00 USD

25 tests
1F-419-T025
In stock
99.00 USD
Variant
100 tests
1F-419-T100
In stock
198.00 USD

25 tests
1F-419-T025
In stock
99.00 USD

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