Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD235ab FITC

Anti-Hu CD235ab FITC

Regulatory status

RUO

Antigen

CD235ab

Clone

HIR2

Format

FITC

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1F-419-T100

In stock

198.00 USD

25 tests

1F-419-T025

In stock

99.00 USD

Variant

100 tests

1F-419-T100

In stock

198.00 USD

25 tests

1F-419-T025

In stock

99.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b

Specificity

The mouse monoclonal antibody HIR2 recognizes an epitope within the N-terminal (extracellular) portion of CD235a (glycophorin A) and CD235b (glycophorin B). Its antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells.

Workshop

HLDA VII: WS Code 70299

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

Synthetic peptide (Human, N-terminal)

Preparation

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Glycophorin A/B, GYPA/B, GPA/B, PAS-2/3, Sialoglycoprotein alpha/beta, GPSAT, GPErik, MSN blood group

Antigen description

CD235a (glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to CD235b (glycophorin B, GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, a Streptococcus adhesin.

Flow cytometry analysis (surface staining) of human peripheral blood with anti-CD235ab (HIR2) FITC.

Product specific references:

Marino JH, Tan C, Taylor AA, Bentley C, Van De Wiele CJ, Ranne R, Paliotta M, Broughan TA, Teague TK: Differential IL-7 responses in developing human thymocytes. Hum Immunol. 2010 Apr;71(4):329-33.
PubMed

Sponaas AM, Moharrami NN, Feyzi E, Standal T, Holth Rustad E, Waage A, Sundan A: PDL1 expression on plasma and dendritic cells in myeloma bone marrow suggests benefit of targeted anti PD1-PDL1 therapy. PLoS One. 2015 Oct 7;10(10):e0139867.
PubMed

Kyttälä A, Moraghebi R, Valensisi C, Kettunen J, Andrus C, Pasumarthy KK, Nakanishi M, Nishimura K, Ohtaka M, Weltner J, Van Handel B, Parkkonen O, Sinisalo J, Jalanko A, Hawkins RD, Woods NB, Otonkoski T, Trokovic R: Genetic variability overrides the impact of parental cell type and determines iPSC differentiation potential.
PubMed

Woo AJ, Kim J, Xu J, Huang H, Cantor AB: Role of ZBP-89 in human globin gene regulation and erythroid differentiation. Blood. 2011 Sep 29;118(13):3684-93.
PubMed