Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD235a Purified

Anti-Hu CD235a Purified

Regulatory status

RUO

Antigen

CD235a

Clone

HIR2

Format

Purified

Reactivity

Human

Application

FC (QC tested), AGG, IHC(F), IHC(P)

Variant

0.1 mg

11-419-C100

In stock

140.00 USD

0.025 mg

11-419-C025

Delivery 1 week

70.00 USD

Variant

0.1 mg

11-261-C100

In stock

140.00 USD

0.1 mg

11-261-C100

In stock

70.00 USD

Contact distributor

Product details

Description

Images

References

Isotype

Mouse IgG2b

Specificity

The mouse monoclonal antibody HIR2 recognizes the N-terminal (extracellular) portion of glycophorin A (and weakly of glycophorin B). Its antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells.

Workshop

HLDA VII: WS Code 70299

Application

FC (QC tested), AGG, IHC(F), IHC(P)

Application details

Flow cytometry: Recommended dilution: 1-4 µg/ml

Reactivity

Human

Immunogen

Synthetic peptide (Human, N-terminal)

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Glycophorin A, GYPA, GPA, PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein, GPSAT, GPErik, MSN blood group

Antigen description

CD235a (Glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to glycophorin B (GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, an Streptococcus adhesin.

Entrez Gene ID 2993

UniProt ID P02724

Flow cytometry analysis (surface staining) of human peripheral blood with anti-CD235a (HIR2) purified, GAM-APC.

Immunohistochemistry staining of human adrenal (paraffin-embedded sections) with anti-CD235a (HIR2), 10 μg/ml. Commercially tested by LifeSpan BioSciences.

General references:

Nakahata T and Okumura N: Cell surface antigen expression in human erythroid progenitors: erythroid and megakaryocytic markers. Leuk Lymphoma. 1994;13: 401.
PubMed

Yajima A, Urano-Tashiro Y, Shimazu K, Takashima E, Takahashi Y, Konishi K: Hsa, an adhesin of Streptococcus gordonii DL1, binds to alpha2-3-linked sialic acid on glycophorin A of the erythrocyte membrane. Microbiol Immunol. 2008;52(2):69-77.
PubMed

Rogers CE, Bradley MS, Palsson BO et al: Flow cytometric analysis of human bone marrow perfusion cultures: erythroid development and relationship withburst-forming units-erythroid. Exp Hematol. 1996; 24: 597.
PubMed

Bain BJ: Leukemia Diagnosis: a guide to the FAB classification. Gower Medical Publishing; 1990.

Keren DF, Hanson CA and Hurtubise PE, eds.: Flow Cytometry and Clinical Diagnosis. Chicago, IL: ASCP Press; 1994.

Product specific references:

Marino JH, Tan C, Taylor AA, Bentley C, Van De Wiele CJ, Ranne R, Paliotta M, Broughan TA, Teague TK: Differential IL-7 responses in developing human thymocytes. Hum Immunol. 2010 Apr;71(4):329-33.
PubMed

Sponaas AM, Moharrami NN, Feyzi E, Standal T, Holth Rustad E, Waage A, Sundan A: PDL1 expression on plasma and dendritic cells in myeloma bone marrow suggests benefit of targeted anti PD1-PDL1 therapy. PLoS One. 2015 Oct 7;10(10):e0139867.
PubMed

Kyttälä A, Moraghebi R, Valensisi C, Kettunen J, Andrus C, Pasumarthy KK, Nakanishi M, Nishimura K, Ohtaka M, Weltner J, Van Handel B, Parkkonen O, Sinisalo J, Jalanko A, Hawkins RD, Woods NB, Otonkoski T, Trokovic R: Genetic variability overrides the impact of parental cell type and determines iPSC differentiation potential.
PubMed

Woo AJ, Kim J, Xu J, Huang H, Cantor AB: Role of ZBP-89 in human globin gene regulation and erythroid differentiation. Blood. 2011 Sep 29;118(13):3684-93.
PubMed

Variant

0.1 mg

11-419-C100

In stock

140.00 USD

0.025 mg

11-419-C025

Delivery 1 week

70.00 USD

Variant

0.1 mg

11-261-C100