Anti-Hu CD235a PE

Anti-Hu CD235a PE
Regulatory status
RUO
Antigen
CD235a
Clone
HIR2
Format
PE
Reactivity
Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
100 tests
1P-419-T100
In stock

25 tests
1P-419-T025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock
Product details
References
Isotype
Mouse IgG2b
Specificity
The antibody HIR2 recognizes N-terminal portion of glycophorin A (and weakly of glycophorin B). Its antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells.
Workshop
HLDA VII: WS Code 70299
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Synthetic peptide (Human, N-terminal)
Other names
Glycophorin A, GYPA, GPA, PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein, GPSAT, GPErik
Preparation
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.

General references:

Nakahata T and Okumura N: Cell surface antigen expression in human erythroid progenitors: erythroid and megakaryocytic markers. Leuk Lymphoma. 1994;13: 401.
PubMed
Yajima A, Urano-Tashiro Y, Shimazu K, Takashima E, Takahashi Y, Konishi K: Hsa, an adhesin of Streptococcus gordonii DL1, binds to alpha2-3-linked sialic acid on glycophorin A of the erythrocyte membrane. Microbiol Immunol. 2008;52(2):69-77.
PubMed
Rogers CE, Bradley MS, Palsson BO et al: Flow cytometric analysis of human bone marrow perfusion cultures: erythroid development and relationship withburst-forming units-erythroid. Exp Hematol. 1996; 24: 597.
PubMed
Bain BJ: Leukemia Diagnosis: a guide to the FAB classification. Gower Medical Publishing; 1990.
Keren DF, Hanson CA and Hurtubise PE, eds.: Flow Cytometry and Clinical Diagnosis. Chicago, IL: ASCP Press; 1994.

Product specific references:

Marino JH, Tan C, Taylor AA, Bentley C, Van De Wiele CJ, Ranne R, Paliotta M, Broughan TA, Teague TK: Differential IL-7 responses in developing human thymocytes. Hum Immunol. 2010 Apr;71(4):329-33.
PubMed
Sponaas AM, Moharrami NN, Feyzi E, Standal T, Holth Rustad E, Waage A, Sundan A: PDL1 Expression on Plasma and Dendritic Cells in Myeloma Bone Marrow Suggests Benefit of Targeted anti PD1-PDL1 Therapy. PLoS One. 2015 Oct 7;10(10):e0139867.
PubMed
Kyttälä A, Moraghebi R, Valensisi C, Kettunen J, Andrus C, Pasumarthy KK, Nakanishi M, Nishimura K, Ohtaka M, Weltner J, Van Handel B, Parkkonen O, Sinisalo J, Jalanko A, Hawkins RD, Woods NB, Otonkoski T, Trokovic R: Genetic Variability Overrides the Impact of Parental Cell Type and Determines iPSC Differentiation Potential.
PubMed
Woo AJ, Kim J, Xu J, Huang H, Cantor AB: Role of ZBP-89 in human globin gene regulation and erythroid differentiation. Blood. 2011 Sep 29;118(13):3684-93.
PubMed
Variant
100 tests
1P-419-T100
In stock

25 tests
1P-419-T025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock