Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD230 / Prion Purified

Anti-Hu CD230 / Prion Purified

Regulatory status

RUO

Antigen

CD230 / Prion

Clone

EM-20

Format

Purified

Reactivity

Human

Application

Variant

0.1 mg

11-593-C100

In stock

140.00 USD

0.025 mg

11-593-C025

Delivery 1 week

70.00 USD

Variant

0.1 mg

11-593-C100

In stock

140.00 USD

0.025 mg

11-593-C025

Delivery 1 week

70.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The mouse monoclonal antibody EM-20 recognizes human prion protein (PrP). Diglycosylated form of PrP has ~ 40 kDa, monoglycosylated form ~ 30 kDa, and nonglycosylated form ~ 19-21 kDa. This antibody is suitable for discrimination between normal cellular prion protein (PrPc) and its conformationally changed form (PrPSc) prion protein.

Application

Application details

Western blotting: Recommended dilution: 0.5 μg/ml, non-reducing conditions are essential.

Reactivity

Human

Immunogen

Recombinant human prion protein

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Major prion protein, PrP, PrP27-30, PrP33-35C, ASCR, CD230, PRNP, CJD, KURU, PRIP, PRPC

Antigen description

CD230 / human prion protein (PrP), also known as PRNP, is a ubiquitously expressed GPI-anchored cell surface glycoprotein associating with lipid raft components and functioning as a signaling molecule. CD230 / PrP plays a role in apoptosis in a cell context-dependent manner, is involved in proliferation of epithelial cells and in distribution of junction-associated proteins in human enterocytes. Conversion of this normal cellular prion protein (PrPc) into an abnormal conformer (PrPSc) is the crucial step associated with triggering the pathogenesis of the prion neurodegenerative disorders, such as the Creutzfeld-Jakob disease (CJD). Whereas PrPc is rich in alpha-helices, the PrPSc form has higher content of beta-sheets and is resistant to proteinase K.

Entrez Gene ID 5621

UniProt ID P04156

Western blotting analysis of Creutzfeld-Jakob disease (CJD) negative (lane 1, 2) and CJD positive (lane 3, 4) human brain material using anti-PrP antibody (clone EM-20). CJD positive patient has proteinase K resistent prion protein. Lane 1, 4: Samples with proteinase K treatment Lane 2, 3: Samples without proteinase K treatment

General references:

Li C, Yu S, Nakamura F, Yin S, Xu J, Petrolla AA, Singh N, Tartakoff A, Abbott DW, Xin W, Sy MS: Binding of pro-prion to filamin A disrupts cytoskeleton and correlates with poor prognosis in pancreatic cancer. J Clin Invest. 2009 Aug 17. pii: 39542. doi: 10.1172/JCI39542. [Epub ahead of print]
PubMed

Ermonval M, Baudry A, Baychelier F, Pradines E, Pietri M, Oda K, Schneider B, Mouillet-Richard S, Launay JM, Kellermann O: PLoS One. 2009 Aug 4;4(8):e6497.
PubMed

Yang Y, Chen L, Pan HZ, Kou Y, Xu CM: Glycosylation modification of human prion protein provokes apoptosis in HeLa cells in vitro. BMB Rep. 2009 Jun 30;42(6):331-7.
PubMed

Segarra C, Lehmann S, Coste J: Prion protein expression and processing in human mononuclear cells: the impact of the codon 129 prion gene polymorphism. PLoS One. 2009 Jun 4;4(6):e5796.
PubMed

Wang X, Dong CF, Shi Q, Shi S, Wang GR, Lei YJ, Xu K, An R, Chen JM, Jiang HY, Tian C, Gao C, Zhao YJ, Han J, Dong XP: Cytosolic prion protein induces apoptosis in human neuronal cell SH-SY5Y via mitochondrial disruption pathway. BMB Rep. 2009 Jul 31;42(7):444-9.
PubMed

Product specific references:

Dvorakova E, Vranac T, Janouskova O, Černilec M, Koren S, Lukan A, Nováková J, Matej R, Holada K, Čurin Šerbec V: Detection of the GPI-anchorless prion protein fragment PrP226* in human brain. BMC Neurol. 2013 Sep 25;13:126.
PubMed