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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Hu CD230 / Prion Purified
Anti-Hu CD230 / Prion Purified
Regulatory status
RUO
Antigen
CD230 / Prion
Clone
EM-20
Format
Purified
Reactivity
Human
Application
WB
Variant
0.1 mg
11-593-C100
Out of stock
140.00 USD
0.025 mg
11-593-C025
Out of stock
70.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD
0.1 mg
11-261-C100
In stock
70.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG2a
Specificity
The mouse monoclonal antibody EM-20 recognizes human prion protein (PrP). Diglycosylated form of PrP has ~ 40 kDa, monoglycosylated form ~ 30 kDa, and nonglycosylated form ~ 19-21 kDa. This antibody is suitable for discrimination between normal cellular prion protein (PrPc) and its conformationally changed form (PrPSc) prion protein.
Application
WB
Application details
Western blotting: Recommended dilution: 0.5 g/ml, non-reducing conditions are essential.
Reactivity
Human
Immunogen
Recombinant human prion protein
Other names
Major prion protein, PrP, PrP27-30, PrP33-35C, ASCR, CD230, PRNP, CJD, KURU, PRIP, PRPC
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Western blotting analysis of Creutzfeld-Jakob disease (CJD) negative (lane 1, 2) and CJD positive (lane 3, 4) human brain material using anti-PrP antibody (clone EM-20). CJD positive patient has proteinase K resistent prion protein. Lane 1, 4: Samples with proteinase K treatment Lane 2, 3: Samples without proteinase K treatment
General references:
Li C, Yu S, Nakamura F, Yin S, Xu J, Petrolla AA, Singh N, Tartakoff A, Abbott DW, Xin W, Sy MS: Binding of pro-prion to filamin A disrupts cytoskeleton and correlates with poor prognosis in pancreatic cancer. J Clin Invest. 2009 Aug 17. pii: 39542. doi: 10.1172/JCI39542. [Epub ahead of print]
PubMed
Ermonval M, Baudry A, Baychelier F, Pradines E, Pietri M, Oda K, Schneider B, Mouillet-Richard S, Launay JM, Kellermann O: PLoS One. 2009 Aug 4;4(8):e6497.
PubMed
Yang Y, Chen L, Pan HZ, Kou Y, Xu CM: Glycosylation modification of human prion protein provokes apoptosis in HeLa cells in vitro. BMB Rep. 2009 Jun 30;42(6):331-7.
PubMed
Segarra C, Lehmann S, Coste J: Prion protein expression and processing in human mononuclear cells: the impact of the codon 129 prion gene polymorphism. PLoS One. 2009 Jun 4;4(6):e5796.
PubMed
Wang X, Dong CF, Shi Q, Shi S, Wang GR, Lei YJ, Xu K, An R, Chen JM, Jiang HY, Tian C, Gao C, Zhao YJ, Han J, Dong XP: Cytosolic prion protein induces apoptosis in human neuronal cell SH-SY5Y via mitochondrial disruption pathway. BMB Rep. 2009 Jul 31;42(7):444-9.
PubMed
Product specific references:
Dvorakova E, Vranac T, Janouskova O, Černilec M, Koren S, Lukan A, Nováková J, Matej R, Holada K, Čurin Šerbec V: Detection of the GPI-anchorless prion protein fragment PrP226* in human brain. BMC Neurol. 2013 Sep 25;13:126.
PubMed
Variant
0.1 mg
11-593-C100
Out of stock
140.00 USD
0.025 mg
11-593-C025
Out of stock
70.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD
0.1 mg
11-261-C100
In stock
70.00 USD
Contact distributor
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