Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD23 PerCP-Cy™5.5

Anti-Hu CD23 PerCP-Cy™5.5

Anti-Hu CD23 PerCP-Cy™5.5
Regulatory status
RUO
Antigen
CD23
Clone
EBVCS-5
Format
PerCP-Cy™5.5
Reactivity
Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
100 tests
T9-689-T100
Ask for delivery term HERE
300.00 USD
25 tests
T9-689-T025
Ask for delivery term HERE
150.00 USD
Variant
0.1 mg
11-261-C100
In stock
300.00 USD
0.1 mg
11-261-C100
In stock
150.00 USD
Contact distributor
Product details
Description
Images
References
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody EBVCS-5 recognizes an extracellular epitope located in the stalk region of human low affinity IgE receptor (CD23) between the 37 and 25 kDa cleavage sites.
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
EBV-transformed human cells
Other names
FCER2, Fc-epsilon-RII, BLAST-2, FCE2, CLEC4J, IGEBF
Preparation
The purified antibody is conjugated with tandem dye PerCP-Cy™5.5 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Cy™ and CyDye™ are registered trademarks of Cytiva.
Other names
FCER2, Fc-epsilon-RII, BLAST-2, FCE2, CLEC4J, IGEBF
Antigen description
CD23 (Fc epsilon RII), the low affinity IgE receptor, is a 45 kDa type II membrane glycoprotein expressed more or less on eosinophils, follicular dendritic cells, Langerhans cells, mature B cells (mainly upon activation), EBV-transformed lymphoblasts, monocytes, and subpopulation of platelets. A soluble form of 37 kDa and other its fragments were also described. CD23 mediates IgE-dependent cytotoxicity by eosinophils and macrophages, and downregulates IgE secretion in response to high levels of IgE, involving release of pro-inflammatory cytokines.
Entrez Gene ID 2208
UniProt ID P06734
T9-689 FC
Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-human CD23 (EBVCS-5) PerCP-CyTM5.5.

Product specific references:

Byrd JC, O'Brien S, Flinn IW, Kipps TJ, Weiss M, Rai K, Lin TS, Woodworth J, Wynne D, Reid J, Molina A, Leigh B, Harris S: Phase 1 study of lumiliximab with detailed pharmacokinetic and pharmacodynamic measurements in patients with relapsed or refractory chronic lymphocytic leukemia. Clin Cancer Res. 2007 Aug 1;13(15 Pt 1):4448-55.
PubMed
Rumi C, Rutella S, Leone G, Bonini S: Fc-RII/CD23 receptor on circulating human eosinophils. Blood. 1998 Apr 1;91(7):2621-2.
PubMed
Ling NR, Stevenson FK, Brown B: Urinary excretion of CD23 antigen in normal individuals and patients with chronic lymphocytic leukaemia (CLL). Clin Exp Immunol. 1991 Dec;86(3):360-6.
PubMed
Yamaoka KA, Arock M, Issaly F, Dugas N, Le Goff L, Kolb JP: Granulocyte macrophage colony stimulating factor induces Fc epsilon RII/CD23 expression on normal human polymorphonuclear neutrophils. Int Immunol. 1996 Apr;8(4):479-90.
PubMed
Belleau JT, Gandhi RK, McPherson HM, Lew DB: Research upregulation of CD23 (FcepsilonRII) expression in human airway smooth muscle cells (huASMC) in response to IL-4, GM-CSF, and IL-4/GM-CSF. Clin Mol Allergy. 2005 May 20;3:6.
PubMed
Variant
100 tests
T9-689-T100
Ask for delivery term HERE
300.00 USD
25 tests
T9-689-T025
Ask for delivery term HERE
150.00 USD
Variant
0.1 mg
11-261-C100
In stock
300.00 USD
0.1 mg
11-261-C100
In stock
150.00 USD
Contact distributor
Datasheet download
SDS download
Bulk requests
Technical support for this product