Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD229 Purified Low Endotoxin

Anti-Hu CD229 Purified Low Endotoxin

Regulatory status

RUO

Antigen

CD229

Clone

HLy9.25

Format

Purified Low Endotoxin

Reactivity

Human

Application

IP, WB, FC (QC tested), ICC, FUNC

Variant

0.1 mg

12-618-C100

In stock

147.40 USD

Variant

0.1 mg

12-618-C100

In stock

147.40 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody HLy9.25 (also known as HLy9.1.25) recognizes an extracellular epitope of CD229 / Ly9, a 100-120 kDa cell surface glycoprotein expressed on T and B cells.

Workshop

HCDM listed

Application

IP, WB, FC (QC tested), ICC, FUNC

Application details

Functional application: Regulation of activation;
Flow cytometry: Recommended dilution: 6-10 μg/ml; positive control: peripheral blood lymphocytes.

Reactivity

Human

Immunogen

CD299-transfected 300.19 pre-B cell line

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

SLAMF3, LY9

Antigen description

CD229 (Ly9) is a cell surface receptor of the CD150 family, which includes also e.g. CD48 and CD224. Receptors of this family regulate cytokine production and cytotoxicity of lymphocytes and NK cells. High levels of CD229 are found on T and B cells, where its expression increases during their maturation. It is absent on granulocytes, bone marrow-derived dendritic cells, platelets and erythrocytes. CD229 has been also reported on mouse monocytes and NK cells. CD229 interacts homophilically through its N-terminal domain and localizes to the contact site between T cells and antigen presenting B cells during antigen-dependent immune synapse formation.

Entrez Gene ID 4063

UniProt ID Q9HBG7

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD229 (HLy9.25) purified antibody (low endotoxin, concentration in sample 16 μg/ml) GAM APC.

Separation of human CD229 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD229 (HLy9.25) purified antibody (low endotoxin, concentration in sample 16 μg/ml) GAM APC.

General references:

Sintes J, Romero X, Marin P, Terhorst C, Engel P: Differential expression of CD150 (SLAM) family receptors by human hematopoietic stem and progenitor cells. Exp Hematol. 2008 Sep;36(9):1199-204.
PubMed

Romero X, Benítez D, March S, Vilella R, Miralpeix M, Engel P: Differential expression of SAP and EAT-2-binding leukocyte cell-surface molecules CD84, CD150 (SLAM), CD229 (Ly9) and CD244 (2B4). Tissue Antigens. 2004 Aug;64(2):132-44.
PubMed

Bund D, Mayr C, Kofler DM, Hallek M, Wendtner CM: Human Ly9 (CD229) as novel tumor-associated antigen (TAA) in chronic lymphocytic leukemia (B-CLL) recognized by autologous CD8+ T cells. Exp Hematol. 2006 Jul;34(7):860-9.
PubMed

Del Valle JM, Engel P, Martín M: The cell surface expression of SAP-binding receptor CD229 is regulated via its interaction with clathrin-associated adaptor complex 2 (AP-2). J Biol Chem. 2003 May 9;278(19):17430-7.
PubMed

Product specific references:

Romero X, Zapater N, Calvo M, Kalko SG, de la Fuente MA, Tovar V, Ockeloen C, Pizcueta P, Engel P: CD229 (Ly9) lymphocyte cell surface receptor interacts homophilically through its N-terminal domain and relocalizes to the immunological synapse. J Immunol. 2005 Jun 1;174(11):7033-42.
PubMed

de la Fuente MA, Tovar V, Villamor N, Zapater N, Pizcueta P, Campo E, Bosch J, Engel P: Molecular characterization and expression of a novel human leukocyte cell-surface marker homologous to mouse Ly-9. Blood. 2001 Jun 1;97(11):3513-20.
PubMed

Martín M, Del Valle JM, Saborit I, Engel P: Identification of Grb2 as a novel binding partner of the signaling lymphocytic activation molecule-associated protein binding receptor CD229. J Immunol. 2005 May 15;174(10):5977-86.
PubMed