Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD226 PE

Anti-Hu CD226 PE

Regulatory status

RUO

Antigen

CD226

Clone

11A8

Format

Reactivity

Non-human primates, Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-926-T100

In stock

242.00 USD

Variant

100 tests

1P-926-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody 11A8 recognizes an extracellular epitope within CD226 transmembrane glycoprotein.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Reactivity

Non-human primates, Human

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

DNAM1, PTA1, TLiSA1

Antigen description

CD226 is a type I transmembrane glycoprotein, which is expressed after activation on NK cells, platelets, monocytes, and some T cells. Its expression is increased in patients suffering from some autoimmune diseases or viral infections. CD226 is being phosphorylated on its cytoplasmic tail and interacts with LFA-1 complex (CD11a/CD18), resulting in calcium-independent intercellular contacts. Ligands for CD226 are CD155 and CD112.

Entrez Gene ID 10666

UniProt ID Q15762

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD226 (11A8) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD226 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD226 (11A8) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Fourcade J, Sun Z, Chauvin JM, Ka M, Davar D, Pagliano O, Wang H, Saada S, Menna C, Amin R, Sander C, Kirkwood JM, Korman AJ, Zarour HM: CD226 opposes TIGIT to disrupt Tregs in melanoma. JCI Insight. 2018 Jul 26;3(14):e121157.
PubMed

Fuchs A, Cella M, Giurisato E, Shaw AS, Colonna M: Cutting edge: CD96 (tactile) promotes NK cell-target cell adhesion by interacting with the poliovirus receptor (CD155). J Immunol. 2004 Apr 1;172(7):3994-8.
PubMed

Deuss FA, Watson GM, Goodall KJ, Leece I, Chatterjee S, Fu Z, Thaysen-Andersen M, Andrews DM, Rossjohn J, Berry R: Structural basis for the recognition of nectin-like protein-5 by the human-activating immune receptor, DNAM-1. J Biol Chem. 2019 Aug 16;294(33):12534-12546.
PubMed

Deng C, Li W, Fei Y, Wang L, Chen Y, Zeng X, Zhang F, Li Y: Imbalance of the CD226/TIGIT immune checkpoint is involved in the pathogenesis of primary biliary cholangitis. Front Immunol. 2020 Jul 24;11:1619.
PubMed