Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD222 Purified

Anti-Hu CD222 Purified

Regulatory status

RUO

Antigen

CD222

Clone

MEM-240

Format

Purified

Reactivity

Human

Application

IP, WB, FC (QC tested)

Variant

0.1 mg

11-790-C100

In stock

0.025 mg

11-790-C025

Out of stock

Variant

0.1 mg

11-261-C100

In stock

0.1 mg

11-261-C100

In stock

Product details

Images

References

Isotype

Mouse IgG1

Specificity

The antibody MEM-238 recognizes an epitope between amino acids 698-1262 of CD222 (IGF2 receptor), a ubiquitously expressed 250 kDa multifunctional type I transmembrane protein. The majority of CD222 is found in the late endosomal/prelysosomal compartment, 5-10% in the plasma membrane and the truncated (220 kDa) form of CD222 is present in human and bovine serum.

Workshop

HLDA VII; WS Code 70641

Application

IP, WB, FC (QC tested)

Reactivity

Human

Immunogen

Recombinant Vaccinia virus encoding CD222.

Other names

IGF2R, MPR1, CIMPR, MPR300, M6P-R

Concentration

1 mg/ml

Preparation

Purified from cell culture supernatant by protein-A affinity chromatography

Formulation

Phosphate buffered saline (PBS) solution with 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.

Flow cytometry detection of CD222 in human peripheral blood with anti-CD222 (MEM-240) purified, GAM-APC.

General references:

Wood RJ, Hulett MD: Cell surface-expressed cation-independent mannose 6-phosphate receptor (CD222) binds enzymatically active heparanase independently of mannose 6-phosphate to promote extracellular matrix degradation. J Biol Chem. 2008 Feb 15;283(7):4165-76.
PubMed

Leukocyte Typing VII., Mason D. et al. (Eds.), Oxford University Press (2002).

Gasanov U, Koina C, Beagley KW, Aitken RJ, Hansbro PM: Identification of the insulin-like growth factor II receptor as a novel receptor for binding and invasion by Listeria monocytogenes. Infect Immun. 2006 Jan;74(1):566-77.
PubMed

Product specific references:

Schiller HB, Szekeres A, Binder BR, Stockinger H, Leksa V: Mannose 6-phosphate/insulin-like growth factor 2 receptor limits cell invasion by controlling alphaVbeta3 integrin expression and proteolytic processing of urokinase-type plasminogen activator receptor. Mol Biol Cell. 2009 Feb;20(3):745-56.
PubMed

Leksa V, Loewe R, Binder B, Schiller HB, Eckerstorfer P, Forster F, Soler-Cardona A, Ondrovicová G, Kutejová E, Steinhuber E, Breuss J, Drach J, Petzelbauer P, Binder BR, Stockinger H: Soluble M6P/IGF2R released by TACE controls angiogenesis via blocking plasminogen activation. Circ Res. 2011 Mar 18;108(6):676-85.
PubMed

Leksa V, Godar S, Cebecauer M, Hilgert I, Breuss J, Weidle UH, Horejsi V, Binder BR, Stockinger H: The N terminus of mannose 6-phosphate / insulin-like growth factor 2 receptor in regulation of fibrinolysis and cell migration. J Biol Chem. 2002 Oct 25;277(43):40575-82.
PubMed

Variant

0.1 mg

11-790-C100

In stock

0.025 mg

11-790-C025

Out of stock

Variant

0.1 mg

11-261-C100

In stock

0.1 mg

11-261-C100

In stock

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