Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD222 Purified

Anti-Hu CD222 Purified

Anti-Hu CD222 Purified
Regulatory status
RUO
Antigen
CD222
Clone
MEM-240
Format
Purified
Reactivity
Human
Application
IP, WB, FC (QC tested)
Variant
0.1 mg
11-790-C100
In stock
150.00 USD
0.025 mg
11-790-C025
Ask for delivery term HERE
75.00 USD
Variant
0.1 mg
11-261-C100
In stock
150.00 USD
0.1 mg
11-261-C100
In stock
75.00 USD
Contact distributor
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The antibody MEM-238 recognizes an extracellular epitope between amino acids 698-1262 of CD222 (IGF2 receptor), a ubiquitously expressed 250 kDa multifunctional type I transmembrane protein. The majority of CD222 is found in the late endosomal/prelysosomal compartment, 5-10% in the plasma membrane and the truncated (220 kDa) form of CD222 is present in human and bovine serum.
Workshop
HLDA VII; WS Code 70641
Application
IP, WB, FC (QC tested)
Application details
Flow cytometry: Extracellular and intracellular staining. Recommended dilution: 2-6 μg/ml.
Reactivity
Human
Immunogen
Recombinant Vaccinia virus encoding CD222.
Other names
IGF2R, MPR1, CIMPR, MPR300, M6P-R
Concentration
1 mg/ml
Preparation
Purified from cell culture supernatant by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
IGF2R, MPR1, CIMPR, MPR300, M6P-R
Antigen description
CD222 (CIMPR, cation-independent mannose 6-phosphate receptor; IGF2 receptor) is a ubiquitously expressed 250 kDa transmembrane protein. No more than 10% of CD222 is present on the cell surface where it serves as a multifunctional receptor. Intracellular (major) fraction of CD222 is involved in transport of newly synthesized lysosomal enzymes modified by mannose 6-phosphate from Golgi apparatus to lysosomes. The cell surface CD222 binds and internalizes exogeneous mannose 6-phosphate-containing ligands. Importantly, CD222 is crutial for internalization and degradation of insulin-like growth factor 2, thus controling cell growth. CD222 also complexes CD87 (urokinase-type plasminogen-activator receptor), plasminogen and latent TGF-beta, last but not least CD222 serves as a receptor for heparanase and even for Listeria.
Entrez Gene ID 3482
UniProt ID P11717
11-790_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD222 (MEM-240) purified antibody (concentration in sample 3 μg/ml) GAM APC.
11-790_FC_Histogram
Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD222 (MEM-240) purified antibody (concentration in sample 3 μg/ml) GAM APC.
11-790 FC
Flow cytometry detection of CD222 in human peripheral blood with anti-CD222 (MEM-240) purified, GAM-APC.

General references:

Wood RJ, Hulett MD: Cell surface-expressed cation-independent mannose 6-phosphate receptor (CD222) binds enzymatically active heparanase independently of mannose 6-phosphate to promote extracellular matrix degradation. J Biol Chem. 2008 Feb 15;283(7):4165-76.
PubMed
Leukocyte Typing VII., Mason D. et al. (Eds.), Oxford University Press (2002).
Gasanov U, Koina C, Beagley KW, Aitken RJ, Hansbro PM: Identification of the insulin-like growth factor II receptor as a novel receptor for binding and invasion by Listeria monocytogenes. Infect Immun. 2006 Jan;74(1):566-77.
PubMed

Product specific references:

Schiller HB, Szekeres A, Binder BR, Stockinger H, Leksa V: Mannose 6-phosphate/insulin-like growth factor 2 receptor limits cell invasion by controlling alphaVbeta3 integrin expression and proteolytic processing of urokinase-type plasminogen activator receptor. Mol Biol Cell. 2009 Feb;20(3):745-56.
PubMed
Leksa V, Loewe R, Binder B, Schiller HB, Eckerstorfer P, Forster F, Soler-Cardona A, Ondrovicová G, Kutejová E, Steinhuber E, Breuss J, Drach J, Petzelbauer P, Binder BR, Stockinger H: Soluble M6P/IGF2R released by TACE controls angiogenesis via blocking plasminogen activation. Circ Res. 2011 Mar 18;108(6):676-85.
PubMed
Leksa V, Godar S, Cebecauer M, Hilgert I, Breuss J, Weidle UH, Horejsi V, Binder BR, Stockinger H: The N terminus of mannose 6-phosphate / insulin-like growth factor 2 receptor in regulation of fibrinolysis and cell migration. J Biol Chem. 2002 Oct 25;277(43):40575-82.
PubMed
Variant
0.1 mg
11-790-C100
In stock
150.00 USD
0.025 mg
11-790-C025
Ask for delivery term HERE
75.00 USD
Variant
0.1 mg
11-261-C100
In stock
150.00 USD
0.1 mg
11-261-C100
In stock
75.00 USD
Contact distributor
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