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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Hu CD222 Biotin
Anti-Hu CD222 Biotin
Regulatory status
RUO
Antigen
CD222
Clone
MEM-238
Format
Biotin
Reactivity
Non-human primates, Human
Application
IP, WB, FC (QC tested)
Variant
0.1 mg
1B-315-C100
In stock
198.00 USD
0.025 mg
1B-315-C025
Delivery 1 week
99.00 USD
Variant
0.1 mg
1B-315-C100
In stock
198.00 USD
0.025 mg
1B-315-C025
Delivery 1 week
99.00 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The antibody MEM-238 recognizes an extracellular epitope between amino acids 192-697 of CD222 (IGF2 receptor), a ubiquitously expressed 250 kDa multifunctional type I transmembrane protein. The majority of CD222 is found in the late endosomal/prelysosomal compartment, 5-10% in the plasma membrane and the truncated (220 kDa) form of CD222 is present in human and bovine serum.
Workshop
HLDA VII; WS Code 70640
Application
IP, WB, FC (QC tested)
Application details
Flow cytometry: Extracellular and intracellular staining; recommended dilution: 2-6 μg/ml.
Western blotting: Recommended dilution: 1 µg/ml; non-reducing conditions.
Reactivity
Non-human primates, Human
Immunogen
Recombinant
Vaccinia
virus encoding CD222.
Concentration
1 mg/ml
Preparation
Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
IGF2R, MPR1, CIMPR, MPR300, M6P-R
Antigen description
CD222 (CIMPR, cation-independent mannose 6-phosphate receptor; IGF2 receptor) is a ubiquitously expressed 250 kDa transmembrane protein. No more than 10% of CD222 is present on the cell surface where it serves as a multifunctional receptor. Intracellular (major) fraction of CD222 is involved in transport of newly synthesized lysosomal enzymes modified by mannose 6-phosphate from Golgi apparatus to lysosomes. The cell surface CD222 binds and internalizes exogeneous mannose 6-phosphate-containing ligands. Importantly, CD222 is crutial for internalization and degradation of insulin-like growth factor 2, thus controling cell growth. CD222 also complexes CD87 (urokinase-type plasminogen-activator receptor), plasminogen and latent TGF-beta, last but not least CD222 serves as a receptor for heparanase and even for Listeria.
Entrez Gene ID
3482
UniProt ID
P11717
Flow cytometry analysis (surface staining) of human peripheral blood with anti-CD222 (MEM-238) biotin, streptavidin-APC.
Anti-Hu CD222 Biotin (clone MEM-238) works in WB application under non-reducing conditions.
Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Raji, Jurkat, HeLa, and K562 cell lines, mixed and heated (100°C, 5 min) with non-reducing SDS-loading buffer. Samples were resolved using 7% SDS-PAGE gel.
Nitrocellulose membrane blot was probed with biotinylated mouse IgG1 monoclonal antibody MEM-238 (1 µg/ml), followed by IRDye 800CW Streptavidin (green). Multiplex fluorescent Western blot detection was performed.
CD222 molecules were detected at ~250 kDa in all analysed cell lines.
General references:
Wood RJ, Hulett MD: Cell surface-expressed cation-independent mannose 6-phosphate receptor (CD222) binds enzymatically active heparanase independently of mannose 6-phosphate to promote extracellular matrix degradation. J Biol Chem. 2008 Feb 15;283(7):4165-76.
PubMed
Leukocyte Typing VII., Mason D. et al. (Eds.), Oxford University Press (2002).
Gasanov U, Koina C, Beagley KW, Aitken RJ, Hansbro PM: Identification of the insulin-like growth factor II receptor as a novel receptor for binding and invasion by Listeria monocytogenes. Infect Immun. 2006 Jan;74(1):566-77.
PubMed
Product specific references:
Machacek C, Supper V, Leksa V, Mitulovic G, Spittler A, Drbal K, Suchanek M, Ohradanova-Repic A, Stockinger H: Folate Receptor β Regulates Integrin CD11b/CD18 Adhesion of a Macrophage Subset to Collagen. J Immunol. 2016 Sep 15;197(6):2229-38.
PubMed
Leksa V, Godar S, Cebecauer M, Hilgert I, Breuss J, Weidle UH, Horejsi V, Binder BR, Stockinger H: The N terminus of mannose 6-phosphate/insulin-like growth factor 2 receptor in regulation of fibrinolysis and cell migration. J Biol Chem. 2002 Oct 25;277(43):40575-82.
PubMed
Schatzlmaier P, Supper V, Göschl L, Zwirzitz A, Eckerstorfer P, Ellmeier W, Huppa JB, Stockinger H: Rapid multiplex analysis of lipid raft components with single-cell resolution. Sci Signal. 2015 Sep 22;8(395):rs11
PubMed
Leksa V, Pfisterer K, Ondrovičová G, Binder B, Lakatošová S, Donner C, Schiller HB, Zwirzitz A, Mrvová K, Pevala V, Kutejová E, Stockinger H: Dissecting mannose 6-phosphate-insulin-like growth factor 2 receptor complexes that control activation and uptake of plasminogen in cells. J Biol Chem. 2012 Jun 29;287(27):22450-62.
PubMed
Schiller HB, Szekeres A, Binder BR, Stockinger H, Leksa V: Mannose 6-phosphate/insulin-like growth factor 2 receptor limits cell invasion by controlling alphaVbeta3 integrin expression and proteolytic processing of urokinase-type plasminogen activator receptor.
PubMed
Leksa V, Loewe R, Binder B, Schiller HB, Eckerstorfer P, Forster F, Soler-Cardona A, Ondrovicová G, Kutejová E, Steinhuber E, Breuss J, Drach J, Petzelbauer P, Binder BR, Stockinger H: Soluble M6P/IGF2R released by TACE controls angiogenesis via blocking plasminogen activation.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v6_SL.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
0.1 mg
1B-315-C100
In stock
198.00 USD
0.025 mg
1B-315-C025
Delivery 1 week
99.00 USD
Variant
0.1 mg
1B-315-C100
In stock
198.00 USD
0.025 mg
1B-315-C025
Delivery 1 week
99.00 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
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