Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD22 Purified Azide Free

Anti-Hu CD22 Purified Azide Free

Anti-Hu CD22 Purified Azide Free
Regulatory status
RUO
Antigen
CD22
Clone
IS7
Format
Purified Azide Free
Reactivity
Human
Application
FC (QC tested)
Variant
0.1 mg
10-506-C100
In stock
130.00 USD
0.025 mg
10-506-C025
Out of stock
65.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD
0.1 mg
11-261-C100
In stock
65.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The antibody IS7 reacts with an extracellular epitope of CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes.
Workshop
HLDA IV: WS Code B 227; HLDA V: WS Code B CD22.8
Application
FC (QC tested)
Application details
Flow cytometry: Recommended dilution: 1-4 µg/ml, Positive control: lysed whole blood; lyse-wash protocol
Reactivity
Human
Immunogen
human cell line Reh
Other names
SIGLEC2, SIGLEC-2
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Azide free phosphate buffered saline (PBS), approx. pH 7.4
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
10-506 FC
Flow cytometry analysis (surface staining) of CD22 in human peripheral blood cells with anti-CD22 (IS7) azide free, GAM-APC.

General references:

Walker JA, Smith KG: CD22: an inhibitory enigma. Immunology. 2007 Dec 7
PubMed
Tateno H, Li H, Schur MJ, Bovin N, Crocker PR, Wakarchuk WW, Paulson JC: Distinct endocytic mechanisms of CD22 (Siglec-2) and Siglec-F reflect roles in cell signaling and innate immunity. Mol Cell Biol. 2007 Aug;27(16):5699-710.
PubMed
Tedder TF, Poe JC, Haas KM: CD22: A Multifunctional Receptor That Regulates B Lymphocyte Survival and Signal Transduction. Adv Immunol. 2005;88:1-50.
PubMed
Variant
0.1 mg
10-506-C100
In stock
130.00 USD
0.025 mg
10-506-C025
Out of stock
65.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD
0.1 mg
11-261-C100
In stock
65.00 USD
Contact distributor
Datasheet download
SDS download
Bulk requests
Technical support for this product