Anti-Hu CD22 Purified

Anti-Hu CD22 Purified
Regulatory status
RUO
Antigen
CD22
Clone
MEM-01
Format
Purified
Reactivity
Human, Non-human primates
Variant
0.1 mg
11-265-C100
In stock
147.40 USD

0.025 mg
11-265-C025
In stock
73.70 USD
Variant
0.1 mg
11-265-C100
In stock
147.40 USD

0.025 mg
11-265-C025
In stock
73.70 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The antibody MEM-01 reacts with an extracellular epitope of CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes. The antibody MEM-01 cross-blocks the antibody OTH228 that recognizes uniquely epitope "E"; it does not cross-block antibodies RFB-4, CLB22/1 and CLB-BLy1.
Application details
Flow cytometry: Recommended dilution: 2-4 μg/ml.
Western blotting: Recommended dilution: 1-2 μg/ml, non-reducing conditions. The antibody MEM-01 stains only the higher Mw isoform (140 kDa, migrating sometimes as a 125 kDa band) and not the lower Mw isoform (130 kDa, migrating sometimes as a 115 kDa band).
Reactivity
Human, Non-human primates
Immunogen
Raji Burkitt's lymphoma cell line
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
SIGLEC2, SIGLEC-2
Antigen description
CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.
Entrez Gene ID 933
UniProt ID P20273
11-265_FC_Profil značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD22 (MEM-01) purified antibody (concentration in sample 0,6 μg/ml, GAM APC).
11-265_FC_Histogram
Separation of human CD22 positive lymphocytes (red-filled) from human CD22 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD22 (MEM-01) purified antibody (concentration in sample 0,6 μg/ml, GAM APC).
11-265_WB-react
Anti-Hu CD22 Purified (clone MEM-01) reactivity pattern in WB application under non-reducing conditions.
The reactivity of MEM-01 antibody was assessed by comparing binding signals in a panel of three human B cell and one T cell line. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Raji, Ramos, Daudi, and Jurkat cell lines, mixed and heated (100°C, 5 min) without reducing agent in SDS-loading buffer. Samples were resolved using 7% SDS-PAGE gel.

Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody MEM-01 (2 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green).

CD22 was detected at ~125 kDa in Raji and Daudi cell line.

General references:

Walker JA, Smith KG: CD22: an inhibitory enigma. Immunology. 2007 Dec 7
PubMed
Tateno H, Li H, Schur MJ, Bovin N, Crocker PR, Wakarchuk WW, Paulson JC: Distinct endocytic mechanisms of CD22 (Siglec-2) and Siglec-F reflect roles in cell signaling and innate immunity. Mol Cell Biol. 2007 Aug;27(16):5699-710.
PubMed
Tedder TF, Poe JC, Haas KM: CD22: A Multifunctional Receptor That Regulates B Lymphocyte Survival and Signal Transduction. Adv Immunol. 2005;88:1-50.
PubMed
Variant
0.1 mg
11-265-C100
In stock
147.40 USD

0.025 mg
11-265-C025
In stock
73.70 USD
Variant
0.1 mg
11-265-C100
In stock
147.40 USD

0.025 mg
11-265-C025
In stock
73.70 USD

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