Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD22 Purified

Anti-Hu CD22 Purified

Regulatory status

RUO

Antigen

CD22

Clone

MEM-01

Format

Purified

Reactivity

Human, Non-human primates

Application

IP, WB, FC (QC tested)

Variant

0.1 mg

11-265-C100

In stock

143.00 USD

0.025 mg

11-265-C025

In stock

71.50 USD

Variant

0.1 mg

11-265-C100

In stock

143.00 USD

0.025 mg

11-265-C025

In stock

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody MEM-01 reacts with an extracellular epitope of CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes. The antibody MEM-01 cross-blocks the antibody OTH228 that recognizes uniquely epitope "E"; it does not cross-block antibodies RFB-4, CLB22/1 and CLB-BLy1.

Application

IP, WB, FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 2-4 μg/ml.
Western blotting: Recommended dilution: 2-4 μg/ml, 120 min on vertical incubator; positive control: RAJI human lymphoma cell line. The antibody MEM-01 stains only the zone corresponding to 140 kDa, but not the weaker 130 kDa zone (stained by several CD22 antibodies recognizing both isoforms).

Reactivity

Human, Non-human primates

Immunogen

Raji Burkitt's lymphoma cell line

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

SIGLEC2, SIGLEC-2

Antigen description

CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.

Entrez Gene ID 933

UniProt ID P20273

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD22 (MEM-01) purified antibody (concentration in sample 0,6 μg/ml, GAM APC).

Separation of human CD22 positive lymphocytes (red-filled) from human CD22 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD22 (MEM-01) purified antibody (concentration in sample 0,6 μg/ml, GAM APC).

General references:

Walker JA, Smith KG: CD22: an inhibitory enigma. Immunology. 2007 Dec 7
PubMed

Tateno H, Li H, Schur MJ, Bovin N, Crocker PR, Wakarchuk WW, Paulson JC: Distinct endocytic mechanisms of CD22 (Siglec-2) and Siglec-F reflect roles in cell signaling and innate immunity. Mol Cell Biol. 2007 Aug;27(16):5699-710.
PubMed

Tedder TF, Poe JC, Haas KM: CD22: A Multifunctional Receptor That Regulates B Lymphocyte Survival and Signal Transduction. Adv Immunol. 2005;88:1-50.
PubMed