Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD22 Purified

Anti-Hu CD22 Purified

Regulatory status

RUO

Antigen

CD22

Clone

S-HCL-1

Format

Purified

Reactivity

Human

Application

WB, FC (QC tested)

Variant

0.1 mg

11-175-C100

Ask for delivery term HERE

143.00 USD

0.025 mg

11-175-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-175-C100

Ask for delivery term HERE

143.00 USD

0.025 mg

11-175-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b kappa

Specificity

The mouse monoclonal antibody S-HCL-1 (also known as S-HCL1) recognizes an extracellular epitope of CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes.

Workshop

HLDA IV: WS Code B48

Application

WB, FC (QC tested)

Application details

Flow cytometry: Extracellular and intracellular staining; recommended dilution: 1-4 μg/ml.
Western blotting: Recommended dilution: 1-2 μg/ml, non-reducing conditions.

Reactivity

Human

Immunogen

Whole hairy cell leukemia cells and membrane preparation

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

SIGLEC2, SIGLEC-2

Antigen description

CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.

Entrez Gene ID 933

UniProt ID P20273

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD22 (S-HCL-1) purified antibody (concentration in sample 3 μg/ml) GAM APC.

Separation of human CD22 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD22 (S-HCL-1) purified antibody (concentration in sample 3 μg/ml) GAM APC.

Anti-Hu CD22 Purified (clone S-HCL-1) reactivity pattern in WB application under non-reducing conditions.
The reactivity of S-HCL-1 antibody was assessed by comparing binding signals in a panel of three human B cell and one T cell line. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Raji, Ramos, Daudi, and Jurkat cell lines, mixed and heated (100°C, 5 min) without reducing agent in SDS-loading buffer. Samples were resolved using 7% SDS-PAGE gel.

Nitrocellulose membrane blot was probed with mouse IgG2b monoclonal antibody S-HCL-1 (2 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green).

CD22 was detected at ~110-125 kDa in Daudi cell line.

General references:

Walker JA, Smith KG: CD22: an inhibitory enigma. Immunology. 2007 Dec 7
PubMed

Tateno H, Li H, Schur MJ, Bovin N, Crocker PR, Wakarchuk WW, Paulson JC: Distinct endocytic mechanisms of CD22 (Siglec-2) and Siglec-F reflect roles in cell signaling and innate immunity. Mol Cell Biol. 2007 Aug;27(16):5699-710.
PubMed

Tedder TF, Poe JC, Haas KM: CD22: A Multifunctional Receptor That Regulates B Lymphocyte Survival and Signal Transduction. Adv Immunol. 2005;88:1-50.
PubMed

Product specific references:

Reineks EZ, Osei ES, Rosenberg A, Auletta J, Meyerson HJ: CD22 expression on blastic plasmacytoid dendritic cell neoplasms and reactivity of anti-CD22 antibodies to peripheral blood dendritic cells. Cytometry B Clin Cytom. 2009 Jul;76(4):237-48
PubMed

James SE, Greenberg PD, Jensen MC, Lin Y, Wang J, Till BG, Raubitschek AA, Forman SJ, Press OW: Antigen sensitivity of CD22-specific chimeric TCR is modulated by target epitope distance from the cell membrane. J Immunol. 2008 May 15;180(10):7028-38.
PubMed

Huang J, Fan G, Zhong Y, Gatter K, Braziel R, Gross G, Bakke A: Diagnostic usefulness of aberrant CD22 expression in differentiating neoplastic cells of B-Cell chronic lymphoproliferative disorders from admixed benign B cells in four-color multiparameter flow cytometry. Am J Clin Pathol. 2005 Jun;123(6):826-32.
PubMed

Foon KA, Todd RF 3rd: Immunologic classification of leukemia and lymphoma. Blood. 1986 Jul;68(1):1-31.
PubMed