Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD218a Purified

Anti-Hu CD218a Purified

Regulatory status

RUO

Antigen

CD218a

Clone

H44

Format

Purified

Reactivity

Human

Application

IP, WB, FC (QC tested), IHC-F, IHC-P

Variant

0.1 mg

11-887-C100

In stock

158.40 USD

Variant

0.1 mg

11-887-C100

In stock

158.40 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody H44 recognizes an extracellular epitope of CD218a, the alpha subunit of IL-18 receptor.

Application

IP, WB, FC (QC tested), IHC-F, IHC-P

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Human

Immunogen

Human CD218a expressed on a transfected NK0 cell line

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

IL18R1, IL-18 R1, IL18-RA

Antigen description

CD218a, an approximately 62 kDa type I transmembrane glycoprotein, is the alpha subunit of IL-18 receptor heterodimer. It is expressed in various immune cells, including lymphocytes, NK cells, and myeloid cells, as well as in heart, lung, liver, gut and another tissues. IL-18 is a proinflammatory cytokine, that promotes both Th1 and Th2 immune responses, induces IFN-gamma production and activates NK cells.

Entrez Gene ID 8809

UniProt ID Q13478

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD218a (H44) purified antibody (concentration in sample 0.5 μg/ml, GAM APC).

Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human CD218a (H44) purified antibody (concentration in sample 0.5 μg/ml, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood).

Separation of human neutrophil granulocytes (red-filled) from monocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD218a (H44) purified antibody (concentration in sample 0.5 μg/ml, GAM APC).

Product specific references:

Wu Z, Valentina V, Chen J, Sun W, Lin Z, Keyvanfar K, Talasani N, Kajigaya S, Feng X, Young NS: Interleukin-18 plays a dispensable role in murine and likely also human bone marrow failure. Exp Hematol. 2019 Jan;69:54-64.e2.
PubMed