Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD209 Purified

Anti-Hu CD209 Purified

Regulatory status

RUO

Antigen

CD209

Clone

UW60.1

Format

Purified

Reactivity

Human

Application

IHC, FC (QC tested), ICC

Variant

0.1 mg

11-640-C100

In stock

143.00 USD

0.025 mg

11-640-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-640-C100

In stock

143.00 USD

0.025 mg

11-640-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The mouse monoclonal antibody UW60.1 recognizes an extracellular epitope of human CD209 (DC-SIGN), a 44 kDa transmembrane receptor, expressed on the surface of dendritic cells and macrophages.

Application

IHC, FC (QC tested), ICC

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Human

Immunogen

CD209-His-tagged fusion protein

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CLEC4L, DC-SIGN1, CDSIGN

Antigen description

CD209, also known as DC-SIGN (dendritic cell-specific ICAM-3-grabbing nonintegrin) is a transmembrane receptor expressed on the surface of dendritic cells and macrophages, which recognizes numerous pathogens ranging from parasites to viruses. Its N-terminal domain is transmembrane, whereas a tandem-repeat neck domain and the C terminal C-type lectin carbohydrate recognition domain have dual function as a pathogen recognition receptor and a cell adhesion receptor. The neck region is responsible for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity. A ligand of CD209 is also CD50 (ICAM-3).

Entrez Gene ID 30835

UniProt ID Q9NNX6

Flow cytometry surface staining pattern of human stimulated (GM-CSF+IL-4) peripheral blood mononuclear cells stained using anti-human CD209 (UW60.1) purified antibody (concentration in sample 0.6 μg/ml, GAM APC).

Separation of human myeloid cells (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF+IL-4) peripheral blood mononuclear cells stained using anti-human CD209 (UW60.1) purified antibody (concentration in sample 0.6 μg/ml, GAM APC).

General references:

Geijtenbeek TB, Engering A, Van Kooyk Y: DC-SIGN, a C-type lectin on dendritic cells that unveils many aspects of dendritic cell biology. J Leukoc Biol. 2002 Jun;71(6):921-31.
PubMed

van Kooyk Y, Geijtenbeek TB: A novel adhesion pathway that regulates dendritic cell trafficking and T cell interactions. Immunol Rev. 2002 Aug;186:47-56.
PubMed

Khoo US, Chan KY, Chan VS, Lin CL: DC-SIGN and L-SIGN: the SIGNs for infection. J Mol Med. 2008 Aug;86(8):861-74.
PubMed

Product specific references:

Ryan EJ, Marshall AJ, Magaletti D, Floyd H, Draves KE, Olson NE, Clark EA: Dendritic cell-associated lectin-1: a novel dendritic cell-associated, C-type lectin-like molecule enhances T cell secretion of IL-4. J Immunol. 2002 Nov 15;169(10):5638-48.
PubMed