Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD209 PerCP-Cy™5.5

Anti-Hu CD209 PerCP-Cy™5.5

Regulatory status

RUO

Antigen

CD209

Clone

UW60.1

Format

PerCP-Cy™5.5

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

T9-640-T100

In stock

338.80 USD

Variant

100 tests

T9-640-T100

In stock

338.80 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The mouse monoclonal antibody UW60.1 recognizes an extracellular epitope of human CD209 (DC-SIGN), a 44 kDa transmembrane receptor, expressed on the surface of dendritic cells and macrophages.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

CD209-His-tagged fusion protein

Preparation

Purified antibody is conjugated with activated tandem dye of activated Peridinin-Chlorophyll Protein-Cyanine 5.5 (PerCP-Cy™5.5) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Cy™ and CyDye™ are registered trademarks of Cytiva.

Other names

CLEC4L, DC-SIGN1, CDSIGN

Antigen description

CD209, also known as DC-SIGN (dendritic cell-specific ICAM-3-grabbing nonintegrin) is a transmembrane receptor expressed on the surface of dendritic cells and macrophages, which recognizes numerous pathogens ranging from parasites to viruses. Its N-terminal domain is transmembrane, whereas a tandem-repeat neck domain and the C terminal C-type lectin carbohydrate recognition domain have dual function as a pathogen recognition receptor and a cell adhesion receptor. The neck region is responsible for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity. A ligand of CD209 is also CD50 (ICAM-3).

Entrez Gene ID 30835

UniProt ID Q9NNX6

Flow cytometry surface staining pattern of human GM-CSF + IL-4 stimulated peripheral blood mononuclear cells stained using anti-human CD209 (UW60.1) PerCP-Cy™5.5 antibody (concentration in sample 0.56 μg/ml).

Flow cytometry multicolor surface staining pattern of human GM-CSF + IL-4 stimulated peripheral blood mononuclear cells stained using anti-human CD11c (BU15) APC antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD209 (UW60.1) PerCP-Cy™5.5 antibody (concentration in sample 0.56 μg/ml).

Separation of differentiated monocytes stained using anti-human CD209 (UW60.1) PerCP-Cy™5.5 antibody (concentration in sample 0.56 μg/ml, red-filled) from differentiated monocytes using mouse IgG2a isotype control (MOPC-173) PerCP-Cy™5.5 antibody (concentration in sample 0.56 μg/ml, same as CD209 PerCP-Cy™5.5 concentration, black-dashed) in flow cytometry analysis (surface staining) of human GM-CSF + IL-4 stimulated peripheral blood mononuclear cells.

General references:

Geijtenbeek TB, Engering A, Van Kooyk Y: DC-SIGN, a C-type lectin on dendritic cells that unveils many aspects of dendritic cell biology. J Leukoc Biol. 2002 Jun;71(6):921-31.
PubMed

van Kooyk Y, Geijtenbeek TB: A novel adhesion pathway that regulates dendritic cell trafficking and T cell interactions. Immunol Rev. 2002 Aug;186:47-56.
PubMed

Khoo US, Chan KY, Chan VS, Lin CL: DC-SIGN and L-SIGN: the SIGNs for infection. J Mol Med. 2008 Aug;86(8):861-74.
PubMed

Product specific references:

Ryan EJ, Marshall AJ, Magaletti D, Floyd H, Draves KE, Olson NE, Clark EA: Dendritic cell-associated lectin-1: a novel dendritic cell-associated, C-type lectin-like molecule enhances T cell secretion of IL-4. J Immunol. 2002 Nov 15;169(10):5638-48.
PubMed