Anti-Hu CD207 PE

Anti-Hu CD207 PE
Regulatory status
RUO
Antigen
CD207
Clone
2G3
Format
PE
Reactivity
Human, Non-human primates
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1P-946-T100
Ask for delivery term HERE
242.00 USD
Variant
100 tests
1P-946-T100
Ask for delivery term HERE
242.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 lambda
Specificity
The mouse monoclonal antibody 2G3 recognizes an extracellular/luminal epitope of CD207, a transmembrane protein selectively expressed by Langerhans cells and by some other dendritic cell subsets. This antibody clone can target vaccine-peptides to dendritic cells.
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Extracellular and intracellular staining.
Reactivity
Human, Non-human primates
Immunogen
Fusion protein of human CD207 extracellular part and IgG Fc fragment
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
CLEC4K, langerin
Antigen description
CD207 is a 40 kDa type II transmembrane glycoprotein of the C-type lectin family. It binds to mannose-bearing glycoproteins and glycolipids of microbial and viral antigens, including HIV gp120. CD207 is expressed only in Langerhans cells, which are immature dendritic cells of the epidermis and mucosa, and its expression decreases during Langerhans cells maturation. Upon antigen binding CD207 localizes to the Birbeck granules, organelles present in the cytoplasm of Langerhans cells and consisting of superimposed membranes, which leads to a nonclassical antigen-processing pathway.
Entrez Gene ID 50489
UniProt ID Q9UJ71
1P-946_FC_Profil Značení
Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD207 (2G3) PE antibody (10 μl reagent per milion cells in 100 μl of cell suspension).
1P-946_FC_Dot-plot
Flow cytometry multicolor surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD207 (2G3) PE antibody (10 μl reagent per milion cells in 100 μl of cell suspension) and anti-human CD11c (BU15) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).
1P-946_FC_Histogram
Separation of human CD207 positive CD11c positive dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4) (red-filled) from non-stimulated lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD207 (2G3) PE antibody (10 μl reagent per milion cells in 100 μl of cell suspension).

Product specific references:

Epaulard O, Adam L, Poux C, Zurawski G, Salabert N, Rosenbaum P, Dereuddre-Bosquet N, Zurawski S, Flamar AL, Oh S, Romain G, Chapon C, Banchereau J, Lévy Y, Le Grand R, Martinon F: Macrophage- and neutrophil-derived TNF-α instructs skin langerhans cells to prime antiviral immune responses. J Immunol. 2014 Sep 1;193(5):2416-26.
PubMed
Adam L, Tchitchek N, Todorova B, Rosenbaum P, Joly C, Poux C, Chapon C, Spetz AL, Ustav M, Le Grand R, Martinon F: Innate molecular and cellular signature in the skin preceding long-lasting T cell responses after electroporated DNA vaccination. J Immunol. 2020 Jun 15;204(12):3375-3388.
PubMed
Variant
100 tests
1P-946-T100
Ask for delivery term HERE
242.00 USD
Variant
100 tests
1P-946-T100
Ask for delivery term HERE
242.00 USD

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