Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD207 PE

Anti-Hu CD207 PE

Regulatory status

RUO

Antigen

CD207

Clone

2G3

Format

Reactivity

Human, Non-human primates

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-946-T100

In stock

242.00 USD

Variant

100 tests

1P-946-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 lambda

Specificity

The mouse monoclonal antibody 2G3 recognizes an extracellular/luminal epitope of CD207, a transmembrane protein selectively expressed by Langerhans cells and by some other dendritic cell subsets. This antibody clone can target vaccine-peptides to dendritic cells.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Extracellular and intracellular staining.

Reactivity

Human, Non-human primates

Immunogen

Fusion protein of human CD207 extracellular part and IgG Fc fragment

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CLEC4K, langerin

Antigen description

CD207 is a 40 kDa type II transmembrane glycoprotein of the C-type lectin family. It binds to mannose-bearing glycoproteins and glycolipids of microbial and viral antigens, including HIV gp120. CD207 is expressed only in Langerhans cells, which are immature dendritic cells of the epidermis and mucosa, and its expression decreases during Langerhans cells maturation. Upon antigen binding CD207 localizes to the Birbeck granules, organelles present in the cytoplasm of Langerhans cells and consisting of superimposed membranes, which leads to a nonclassical antigen-processing pathway.

Entrez Gene ID 50489

UniProt ID Q9UJ71

Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD207 (2G3) PE antibody (10 μl reagent per milion cells in 100 μl of cell suspension).

Flow cytometry multicolor surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD207 (2G3) PE antibody (10 μl reagent per milion cells in 100 μl of cell suspension) and anti-human CD11c (BU15) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).

Separation of human CD207 positive CD11c positive dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4) (red-filled) from non-stimulated lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD207 (2G3) PE antibody (10 μl reagent per milion cells in 100 μl of cell suspension).

Product specific references:

Epaulard O, Adam L, Poux C, Zurawski G, Salabert N, Rosenbaum P, Dereuddre-Bosquet N, Zurawski S, Flamar AL, Oh S, Romain G, Chapon C, Banchereau J, Lévy Y, Le Grand R, Martinon F: Macrophage- and neutrophil-derived TNF-α instructs skin langerhans cells to prime antiviral immune responses. J Immunol. 2014 Sep 1;193(5):2416-26.
PubMed

Adam L, Tchitchek N, Todorova B, Rosenbaum P, Joly C, Poux C, Chapon C, Spetz AL, Ustav M, Le Grand R, Martinon F: Innate molecular and cellular signature in the skin preceding long-lasting T cell responses after electroporated DNA vaccination. J Immunol. 2020 Jun 15;204(12):3375-3388.
PubMed