Anti-Hu CD207 Alexa Fluor® 647

Anti-Hu CD207 Alexa Fluor<sup>®</sup> 647
Regulatory status
RUO
Antigen
CD207
Clone
2G3
Format
Alexa Fluor® 647
Reactivity
Human, Non-human primates
Excitation laser
red (633 nm)
Variant
100 tests
A6-946-T100
In stock
248.60 USD
Variant
100 tests
A6-946-T100
In stock
248.60 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 lambda
Specificity
The mouse monoclonal antibody 2G3 recognizes an extracellular/luminal epitope of CD207, a transmembrane protein selectively expressed by Langerhans cells and by some other dendritic cell subsets. This antibody clone can target vaccine-peptides to dendritic cells.
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human, Non-human primates
Immunogen
Fusion protein of human CD207 extracellular part and IgG Fc fragment
Preparation
Purified antibody is conjugated with Alexa Fluor® 647 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Other names
CLEC4K, langerin
Antigen description
CD207 is a 40 kDa type II transmembrane glycoprotein of the C-type lectin family. It binds to mannose-bearing glycoproteins and glycolipids of microbial and viral antigens, including HIV gp120. CD207 is expressed only in Langerhans cells, which are immature dendritic cells of the epidermis and mucosa, and its expression decreases during Langerhans cells maturation. Upon antigen binding CD207 localizes to the Birbeck granules, organelles present in the cytoplasm of Langerhans cells and consisting of superimposed membranes, which leads to a nonclassical antigen-processing pathway.
Entrez Gene ID 50489
UniProt ID Q9UJ71
A6-946_FC_Profil Značení
Flow cytometry surface staining pattern of stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD207 (2G3) Alexa Fluor® 647 antibody (4 μl reagent per million cells in 100 μl of cell suspension, red-filled).
A6-946_FC_Dot-plot
Flow cytometry multicolor surface staining of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD207 (2G3) Alexa Fluor® 647 antibody (4 μl reagent per million cells in 100 μl of cell suspension) and anti-human CD11c (BU15) PE antibody (20 μl reagent per milion cells in 100 μl of cell suspension).
A6-946_FC_Histogram
Separation of human CD11c positive CD207 positive differentiated dendritic cells (red-filled) from CD11c negative CD207 negative cell (black-dashed) in flow cytometry analysis (surface staining) of stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD207 (2G3) Alexa Fluor® 647 antibody (4 μl reagent per million cells in 100 μl of cell suspension, red-filled).

Product specific references:

Epaulard O, Adam L, Poux C, Zurawski G, Salabert N, Rosenbaum P, Dereuddre-Bosquet N, Zurawski S, Flamar AL, Oh S, Romain G, Chapon C, Banchereau J, Lévy Y, Le Grand R, Martinon F: Macrophage- and neutrophil-derived TNF-α instructs skin langerhans cells to prime antiviral immune responses. J Immunol. 2014 Sep 1;193(5):2416-26.
PubMed
Adam L, Tchitchek N, Todorova B, Rosenbaum P, Joly C, Poux C, Chapon C, Spetz AL, Ustav M, Le Grand R, Martinon F: Innate molecular and cellular signature in the skin preceding long-lasting T cell responses after electroporated DNA vaccination. J Immunol. 2020 Jun 15;204(12):3375-3388.
PubMed
Variant
100 tests
A6-946-T100
In stock
248.60 USD
Variant
100 tests
A6-946-T100
In stock
248.60 USD

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