Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD206 FITC

Anti-Hu CD206 FITC

Regulatory status

RUO

Antigen

CD206

Clone

15-2

Format

FITC

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1F-782-T100

In stock

198.00 USD

Variant

100 tests

1F-782-T100

In stock

198.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody 15-2 (also known as MR15-2) recognizes an extracellular epitope of CD206 (macrophage mannose receptor, MMR), a 162-175 kDa type I transmembrane protein expressed mainly on macrophages, dendritic cells and hepatic or lymphatic endothelial cells, but not on monocytes.

Workshop

HLDA VII: WS Code 70802

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

Purified human mannose receptor

Preparation

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

MMR, MRC1, CLEC13DL

Antigen description

CD206 (macrophage mannose receptor, MMR), also known as mannose receptor C1 (MRC1), is a type I transmembrane glycoprotein serving as pattern recognition receptor for carbogydrate groups on the surface of bacteria, fungi and other pathogens. Expressed mainly on tissue macrophages and dendritic cells, CD206 mediates endocytosis of these pathogens and presentation of their antigens to the adaptive immune system. CD206 can also be detected in a soluble form in human plasma and is elevated in patients with acute sepsis.

Entrez Gene ID 4360

UniProt ID P22897

Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD206 (15-2) FITC antibody (4 μl reagent per milion cells in 100 μl of cell suspension).

Flow cytometry multicolor surface staining of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD206 (15-2) FITC antibody (4 μl reagent per milion cells in 100 μl of cell suspension) and anti-human CD11c (BU15) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).

Separation of human CD206 positive CD11c positive dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4) (red-filled) from non-stimulated lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD206 (15-2) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Shan M, Klasse PJ, Banerjee K, Dey AK, Iyer SP, Dionisio R, Charles D, Campbell-Gardener L, Olson WC, Sanders RW, Moore JP: HIV-1 gp120 mannoses induce immunosuppressive responses from dendritic cells. PLoS Pathog. 2007 Nov;3(11):e169.
PubMed

Sindrilaru A, Peters T, Wieschalka S, Baican C, Baican A, Peter H, Hainzl A, Schatz S, Qi Y, Schlecht A, Weiss JM, Wlaschek M, Sunderkötter C, Scharffetter-Kochanek K: An unrestrained proinflammatory M1 macrophage population induced by iron impairs wound healing in humans and mice. J Clin Invest. 2011 Mar;121(3):985-97.
PubMed

Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Martinez Casals A, Tian H, Lowery L, Neumann EK, Björklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Mueller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Börner K, Radtke AJ: Organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging. Nat Methods 2023 Aug;20(8):1174-1178. doi: 10.1038/s41592-023-01846-7.
PubMed

Torrelles JB, Azad AK, Schlesinger LS: Fine discrimination in the recognition of individual species of phosphatidyl-myo-inositol mannosides from Mycobacterium tuberculosis by C-type lectin pattern recognition receptors. J Immunol. 2006 Aug 1;177(3):1805-16.
PubMed

Sturge J, Todd SK, Kogianni G, McCarthy A, Isacke CM: Mannose receptor regulation of macrophage cell migration. J Leukoc Biol. 2007 Sep;82(3):585-93.
PubMed

Chang YC, Hsu TL, Lin HH, Chio CC, Chiu AW, Chen NJ, Lin CH, Hsieh SL: Modulation of macrophage differentiation and activation by decoy receptor 3. J Leukoc Biol. 2004 Mar;75(3):486-94.
PubMed