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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Hu CD206 Alexa Fluor<sup>®</sup> 700
Anti-Hu CD206 Alexa Fluor
®
700
Regulatory status
RUO
Antigen
CD206
Clone
15-2
Format
Alexa Fluor
®
700
Reactivity
Human
Application
FC (QC tested)
Excitation laser
red (633 nm)
Variant
100 tests
A7-782-T100
In stock
220.00 USD
Variant
100 tests
A7-782-T100
In stock
220.00 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody 15-2 (also known as MR15-2) recognizes an extracellular epitope of CD206 (macrophage mannose receptor, MMR), a 162-175 kDa type I transmembrane protein expressed mainly on macrophages, dendritic cells and hepatic or lymphatic endothelial cells, but not on monocytes.
Workshop
HLDA VII: WS Code 70802
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10
6
cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Purified human mannose receptor
Preparation
Purified antibody is conjugated with Alexa Fluor
®
700 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor
®
, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Other names
MMR, MRC1, CLEC13DL
Antigen description
CD206 (macrophage mannose receptor, MMR), also known as mannose receptor C1 (MRC1), is a type I transmembrane glycoprotein serving as pattern recognition receptor for carbogydrate groups on the surface of bacteria, fungi and other pathogens. Expressed mainly on tissue macrophages and dendritic cells, CD206 mediates endocytosis of these pathogens and presentation of their antigens to the adaptive immune system. CD206 can also be detected in a soluble form in human plasma and is elevated in patients with acute sepsis.
Entrez Gene ID
4360
UniProt ID
P22897
Flow cytometry surface staining pattern of stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD206 (15.2) Alexa Fluor® 700 antibody (4 μl reagent per million cells in 100 μl of cell suspension, red-filled).
Separation of human differentiated dendritic cells stained using anti-human CD206 (15.2) Alexa Fluor® 700 antibody (4 μl reagent per million cells in 100 μl of cell suspension, red-filled) from differentiated dendritic cells stained using mouse IgG1 isotype control (MOPC-21) Alexa Fluor® 700 antibody (concentration in sample 10 μg/ml, same as CD206 Alexa Fluor® 700 concentration, black-dashed) in flow cytometry analysis (surface staining) of stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells .
Product specific references:
Shan M, Klasse PJ, Banerjee K, Dey AK, Iyer SP, Dionisio R, Charles D, Campbell-Gardener L, Olson WC, Sanders RW, Moore JP: HIV-1 gp120 mannoses induce immunosuppressive responses from dendritic cells. PLoS Pathog. 2007 Nov;3(11):e169.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
Variant
100 tests
A7-782-T100
In stock
220.00 USD
Variant
100 tests
A7-782-T100
In stock
220.00 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
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