Anti-Hu CD206 Alexa Fluor® 647

Anti-Hu CD206 Alexa Fluor® 647
Regulatory status
RUO
Antigen
CD206
Clone
15-2
Format
Alexa Fluor® 647
Reactivity
Human
Application
WB, FC (QC tested), ICC
Excitation laser
red (633 nm)
Variant
100 tests
A6-782-T100
In stock
Variant
0.1 mg
11-261-C100
In stock
Product details
Images
References
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody 15-2 (also known as MR15-2) recognizes an extracellular epitope of CD206 (macrophage mannose receptor, MMR), a 162-175 kDa type I transmembrane protein expressed mainly on macrophages, dendritic cells and hepatic or lymphatic endothelial cells, but not on monocytes.
Workshop
HLDA VII: WS Code 70802
Application
WB, FC (QC tested), ICC
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Purified human mannose receptor
Other names
MMR, MRC1, CLEC13DL
Preparation
The purified antibody is conjugated with Alexa Fluor® 647 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchsing a license to this product for purposes orther than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
A6-782_FC_Profil Značení
Flow cytometry surface staining pattern of human stimulated monocytes (GM-CSF + IL-4) stained using anti-human CD206 (15-2) Alexa Fluor® 647 antibody (4 μl reagent per milion cells in 100 μl of cell suspension).
A6-782_FC_Dot-plot
Flow cytometry multicolor surface staining of human human stimulated monocytes (GM-CSF + IL-4) stained using anti-human CD206 (15-2) Alexa Fluor® 647 antibody (4 μl reagent per milion cells in 100 μl of cell suspension) and anti-human CD11c (BU15) PE antibody (20 μl reagent per milion cells in 100 μl of cell suspension).
A6-782_FC_Histogram
Separation of human CD206 positive CD11c positive dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4) (red-filled) from non-stimulated lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human stimulated monocytes (GM-CSF + IL-4) stained using anti-human CD206 (15-2) Alexa Fluor® 647 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Shan M, Klasse PJ, Banerjee K, Dey AK, Iyer SP, Dionisio R, Charles D, Campbell-Gardener L, Olson WC, Sanders RW, Moore JP: HIV-1 gp120 mannoses induce immunosuppressive responses from dendritic cells. PLoS Pathog. 2007 Nov;3(11):e169.
PubMed
Variant
100 tests
A6-782-T100
In stock
Variant
0.1 mg
11-261-C100
In stock