Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD205 Purified

Anti-Hu CD205 Purified

Anti-Hu CD205 Purified
Regulatory status
RUO
Antigen
CD205
Clone
HD30
Format
Purified
Reactivity
Human
Application
WB, FC (QC tested), IHC(F)
Variant
0.1 mg
11-669-C100
In stock
140.00 USD
0.025 mg
11-669-C025
Delivery 1 week
70.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD
0.1 mg
11-261-C100
In stock
70.00 USD
Contact distributor
Product details
Description
Images
References
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody HD30 recognizes an extracellular epitope of CD205, an approx. 200 kDa C-type lectin transmembrane protein of the MMR (macrophage mannose receptor) family, expressed at high levels on dendritic cells and thymic epithelial cells, and at low levels on lymphocytes, NK cells and monocytes.
Application
WB, FC (QC tested), IHC(F)
Application details
Western blotting: Only non-denatured samples.
Flow cytometry: Recommended dilution: 1-4 µg/ml
Reactivity
Human
Immunogen
Recombinant Fc-tagged human CD205
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
DEC-205, LY75, CLEC13B
Antigen description
CD205, also known as DEC-205, is an endocytic receptor of macrophage mannose receptor family. This 205 kDa C-type lectin transmembrane protein mediates adsorptive uptake and its intracellular domain contains coated pit localization sequence and distal acidic motif, which is required for recycling beyond early endosomes through deeper MHC II+ late endosomes and lysosomes. This unique pathway of receptor-mediated uptake proves to be necessary for presentation of antigenic peptides at low doses of ligand. CD205 is responsible for uptake and processing of captured antigens for dendritic cells.
Entrez Gene ID 4065
UniProt ID O60449
11-669_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD205 (HD30) purified antibody (concentration in sample 0,6 μg/ml, GAM APC).
11-669_FC_Histogram
Separation of human monocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD205 (HD30) purified antibody (concentration in sample 0,6 μg/ml, GAM APC).

General references:

Mahnke K, Guo M, Lee S, Sepulveda H, Swain SL, Nussenzweig M, Steinman RM: The dendritic cell receptor for endocytosis, DEC-205, can recycle and enhance antigen presentation via major histocompatibility complex class II-positive lysosomal compartments. J Cell Biol. 2000 Oct 30;151(3):673-84.
PubMed
Guo M, Gong S, Maric S, Misulovin Z, Pack M, Mahnke K, Nussenzweig MC, Steinman RM: A monoclonal antibody to the DEC-205 endocytosis receptor on human dendritic cells. Hum Immunol. 2000 Aug;61(8):729-38.
PubMed
Gurer C, Strowig T, Brilot F, Pack M, Trumpfheller C, Arrey F, Park CG, Steinman RM, Münz C: Targeting the nuclear antigen 1 of Epstein-Barr virus to the human endocytic receptor DEC-205 stimulates protective T-cell responses. Blood. 2008 Aug 15;112(4):1231-9.
PubMed

Product specific references:

Park CG, Rodriguez A, Ueta H, Lee H, Pack M, Matsuno K, Steinman RM: Generation of anti-human DEC205/CD205 monoclonal antibodies that recognize epitopes conserved in different mammals. 2012 Mar 30;377(1-2):15-22.
PubMed
Variant
0.1 mg
11-669-C100
In stock
140.00 USD
0.025 mg
11-669-C025
Delivery 1 week
70.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD
0.1 mg
11-261-C100
In stock
70.00 USD
Contact distributor
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