Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD205 Purified

Anti-Hu CD205 Purified

Regulatory status

RUO

Antigen

CD205

Clone

HD30

Format

Purified

Reactivity

Human

Application

WB, FC (QC tested), IHC(F)

Variant

0.1 mg

11-669-C100

In stock

140.00 USD

0.025 mg

11-669-C025

Delivery 1 week

70.00 USD

Variant

0.1 mg

11-261-C100

In stock

140.00 USD

0.1 mg

11-261-C100

In stock

70.00 USD

Contact distributor

Product details

Description

Images

References

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody HD30 recognizes an extracellular epitope of CD205, an approx. 200 kDa C-type lectin transmembrane protein of the MMR (macrophage mannose receptor) family, expressed at high levels on dendritic cells and thymic epithelial cells, and at low levels on lymphocytes, NK cells and monocytes.

Application

WB, FC (QC tested), IHC(F)

Application details

Western blotting: Only non-denatured samples.
Flow cytometry: Recommended dilution: 1-4 µg/ml

Reactivity

Human

Immunogen

Recombinant Fc-tagged human CD205

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

DEC-205, LY75, CLEC13B

Antigen description

CD205, also known as DEC-205, is an endocytic receptor of macrophage mannose receptor family. This 205 kDa C-type lectin transmembrane protein mediates adsorptive uptake and its intracellular domain contains coated pit localization sequence and distal acidic motif, which is required for recycling beyond early endosomes through deeper MHC II+ late endosomes and lysosomes. This unique pathway of receptor-mediated uptake proves to be necessary for presentation of antigenic peptides at low doses of ligand. CD205 is responsible for uptake and processing of captured antigens for dendritic cells.

Entrez Gene ID 4065

UniProt ID O60449

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD205 (HD30) purified antibody (concentration in sample 0,6 μg/ml, GAM APC).

Separation of human monocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD205 (HD30) purified antibody (concentration in sample 0,6 μg/ml, GAM APC).

General references:

Mahnke K, Guo M, Lee S, Sepulveda H, Swain SL, Nussenzweig M, Steinman RM: The dendritic cell receptor for endocytosis, DEC-205, can recycle and enhance antigen presentation via major histocompatibility complex class II-positive lysosomal compartments. J Cell Biol. 2000 Oct 30;151(3):673-84.
PubMed

Guo M, Gong S, Maric S, Misulovin Z, Pack M, Mahnke K, Nussenzweig MC, Steinman RM: A monoclonal antibody to the DEC-205 endocytosis receptor on human dendritic cells. Hum Immunol. 2000 Aug;61(8):729-38.
PubMed

Gurer C, Strowig T, Brilot F, Pack M, Trumpfheller C, Arrey F, Park CG, Steinman RM, Münz C: Targeting the nuclear antigen 1 of Epstein-Barr virus to the human endocytic receptor DEC-205 stimulates protective T-cell responses. Blood. 2008 Aug 15;112(4):1231-9.
PubMed

Product specific references:

Park CG, Rodriguez A, Ueta H, Lee H, Pack M, Matsuno K, Steinman RM: Generation of anti-human DEC205/CD205 monoclonal antibodies that recognize epitopes conserved in different mammals. 2012 Mar 30;377(1-2):15-22.
PubMed

Variant

0.1 mg

11-669-C100

In stock

140.00 USD

0.025 mg

11-669-C025

Delivery 1 week

70.00 USD

Variant

0.1 mg

11-261-C100