Anti-Hu CD200R Purified

Anti-Hu CD200R Purified
Regulatory status
RUO
Antigen
CD200R
Clone
OX-108
Format
Purified
Reactivity
Human
Application
FC (QC tested), ICC
Variant
0.1 mg
11-893-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody OX-108 recognizes an extracellular epitope on human CD200R, a transmembrane flycoprotein expressed on the surface of myeloid cells.
Application
FC (QC tested), ICC
Application details
Flow cytometry: Recommended dilution: 2-5 μg/ml.
Reactivity
Human
Immunogen
Recombinant human CD200R
Other names
OX2R, MOX2R, HCRTR2
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
OX2R, MOX2R, HCRTR2
Antigen description
CD200R is a transmembrane glycoprotein, expressed on the surface of myeloid cells. Its interaction with CD200 leads in these cells to a downregulatory signal. This interaction may control myeloid function in a tissue-specific manner. Alternative splicing of CD200R gene results in multiple transcript variants. These isoforms may play a role in differentiation, e.g. regards tolerogenic dendritic cells. Besides myeloid cells, CD200R can be found also on a T cell subset.
Entrez Gene ID 131450
UniProt ID Q8TD46
11-893_FC_profil značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD200R (OX-108) purified antibody (concentration in sample 5 μg/ml, GAM APC).
11-893_FC_Histogram
Separation of leukocytes stained anti-human CD200R (OX-108) purified antibody (concentration in sample 5 μg/ml, GAM APC, red-filled) from leukocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

Product specific references:

Choueiry F, Torok M, Shakya R, Agrawal K, Deems A, Benner B, Hinton A, Shaffer J, Blaser BW, Noonan AM, Williams TM, Dillhoff M, Conwell DL, Hart PA, Cruz-Monserrate Z, Bai XF, Carson WE, Mace TA: CD200 promotes immunosuppression in the pancreatic tumor microenvironment. J Immunother Cancer. 2020 Jun;8(1):e000189.
PubMed
Koning N, Swaab DF, Hoek RM, Huitinga I: Distribution of the immune inhibitory molecules CD200 and CD200R in the normal central nervous system and multiple sclerosis lesions suggests neuron-glia and glia-glia interactions. J Neuropathol Exp Neurol. 2009 Feb;68(2):159-67.
PubMed
Byrareddy SN, Little D, Mayne AE, Villinger F, Ansari AA: Phenotypic and functional characterization of monoclonal antibodies with specificity for rhesus macaque CD200, CD200R and mincle. PLoS One. 2015 Oct 15;10(10):e0140689.
PubMed
Foster-Cuevas M, Wright GJ, Puklavec MJ, Brown MH, Barclay AN: Human herpesvirus 8 K14 protein mimics CD200 in down-regulating macrophage activation through CD200 receptor. J Virol. 2004 Jul;78(14):7667-76.
PubMed
Mihrshahi R, Barclay AN, Brown MH: Essential roles for Dok2 and RasGAP in CD200 receptor-mediated regulation of human myeloid cells. J Immunol. 2009 Oct 15;183(8):4879-86.
PubMed
Misstear K, Chanas SA, Rezaee SA, Colman R, Quinn LL, Long HM, Goodyear O, Lord JM, Hislop AD, Blackbourn DJ: Suppression of antigen-specific T cell responses by the Kaposi's sarcoma-associated herpesvirus viral OX2 protein and its cellular orthologue, CD200. J Virol. 2012 Jun;86(11):6246-57.
PubMed
Letarte M, Voulgaraki D, Hatherley D, Foster-Cuevas M, Saunders NJ, Barclay AN: Analysis of leukocyte membrane protein interactions using protein microarrays. BMC Biochem. 2005 Mar 1;6:2. doi: 10.1186/1471-2091-6-2.
PubMed
Variant
0.1 mg
11-893-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD