Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD200R PE

Anti-Hu CD200R PE

Regulatory status

RUO

Antigen

CD200R

Clone

OX-108

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-893-T100

In stock

242.00 USD

Variant

100 tests

1P-893-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody OX-108 recognizes an extracellular epitope on human CD200R, a transmembrane flycoprotein expressed on the surface of myeloid cells.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

Recombinant human CD200R

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

OX2R, MOX2R, HCRTR2

Antigen description

CD200R is a transmembrane glycoprotein, expressed on the surface of myeloid cells. Its interaction with CD200 leads in these cells to a downregulatory signal. This interaction may control myeloid function in a tissue-specific manner. Alternative splicing of CD200R gene results in multiple transcript variants. These isoforms may play a role in differentiation, e.g. regards tolerogenic dendritic cells. Besides myeloid cells, CD200R can be found also on a T cell subset.

Entrez Gene ID 131450

UniProt ID Q8TD46

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD200R (OX-108) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD200R positive basophil granulocytes (red-filled) from CD200R negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD200R (OX-108) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Choueiry F, Torok M, Shakya R, Agrawal K, Deems A, Benner B, Hinton A, Shaffer J, Blaser BW, Noonan AM, Williams TM, Dillhoff M, Conwell DL, Hart PA, Cruz-Monserrate Z, Bai XF, Carson WE, Mace TA: CD200 promotes immunosuppression in the pancreatic tumor microenvironment. J Immunother Cancer. 2020 Jun;8(1):e000189.
PubMed

Koning N, Swaab DF, Hoek RM, Huitinga I: Distribution of the immune inhibitory molecules CD200 and CD200R in the normal central nervous system and multiple sclerosis lesions suggests neuron-glia and glia-glia interactions. J Neuropathol Exp Neurol. 2009 Feb;68(2):159-67.
PubMed

Byrareddy SN, Little D, Mayne AE, Villinger F, Ansari AA: Phenotypic and functional characterization of monoclonal antibodies with specificity for rhesus macaque CD200, CD200R and mincle. PLoS One. 2015 Oct 15;10(10):e0140689.
PubMed

Foster-Cuevas M, Wright GJ, Puklavec MJ, Brown MH, Barclay AN: Human herpesvirus 8 K14 protein mimics CD200 in down-regulating macrophage activation through CD200 receptor. J Virol. 2004 Jul;78(14):7667-76.
PubMed

Mihrshahi R, Barclay AN, Brown MH: Essential roles for Dok2 and RasGAP in CD200 receptor-mediated regulation of human myeloid cells. J Immunol. 2009 Oct 15;183(8):4879-86.
PubMed

Misstear K, Chanas SA, Rezaee SA, Colman R, Quinn LL, Long HM, Goodyear O, Lord JM, Hislop AD, Blackbourn DJ: Suppression of antigen-specific T cell responses by the Kaposi's sarcoma-associated herpesvirus viral OX2 protein and its cellular orthologue, CD200. J Virol. 2012 Jun;86(11):6246-57.
PubMed

Letarte M, Voulgaraki D, Hatherley D, Foster-Cuevas M, Saunders NJ, Barclay AN: Analysis of leukocyte membrane protein interactions using protein microarrays. BMC Biochem. 2005 Mar 1;6:2. doi: 10.1186/1471-2091-6-2.
PubMed