Anti-Hu CD200R Alexa Fluor® 647

Anti-Hu CD200R Alexa Fluor<sup>®</sup> 647
Regulatory status
RUO
Antigen
CD200R
Clone
OX-108
Format
Alexa Fluor® 647
Reactivity
Human
Excitation laser
red (633 nm)
Variant
100 tests
A6-893-T100
In stock
242.00 USD
Variant
100 tests
A6-893-T100
In stock
242.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody OX-108 recognizes an extracellular epitope on human CD200R, a transmembrane flycoprotein expressed on the surface of myeloid cells.
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Recombinant human CD200R
Preparation
Purified antibody is conjugated with Alexa Fluor® 647 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Other names
OX2R, MOX2R, HCRTR2
Antigen description
CD200R is a transmembrane glycoprotein, expressed on the surface of myeloid cells. Its interaction with CD200 leads in these cells to a downregulatory signal. This interaction may control myeloid function in a tissue-specific manner. Alternative splicing of CD200R gene results in multiple transcript variants. These isoforms may play a role in differentiation, e.g. regards tolerogenic dendritic cells. Besides myeloid cells, CD200R can be found also on a T cell subset.
Entrez Gene ID 131450
UniProt ID Q8TD46
A6-893_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD200R (OX-108) Alexa Fluor® 647 antibody (concentration in sample 0.56 μg/ml).
A6-893_FC_Dot-plot
Flow cytometry multicolor surface staining pattern of human peripheral blood SSC-low events stained using anti-human CD45 (2D1) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood) and anti-human CD200R (OX-108) Alexa Fluor® 647 antibody (concentration in sample 0.56 μg/ml).
A6-893_FC_Histogram
Separation of human CD200R positive CD45dim basophils (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD200R (OX-108) Alexa Fluor® 647 antibody (concentration in sample 0.56 μg/ml).

Product specific references:

Choueiry F, Torok M, Shakya R, Agrawal K, Deems A, Benner B, Hinton A, Shaffer J, Blaser BW, Noonan AM, Williams TM, Dillhoff M, Conwell DL, Hart PA, Cruz-Monserrate Z, Bai XF, Carson WE, Mace TA: CD200 promotes immunosuppression in the pancreatic tumor microenvironment. J Immunother Cancer. 2020 Jun;8(1):e000189.
PubMed
Koning N, Swaab DF, Hoek RM, Huitinga I: Distribution of the immune inhibitory molecules CD200 and CD200R in the normal central nervous system and multiple sclerosis lesions suggests neuron-glia and glia-glia interactions. J Neuropathol Exp Neurol. 2009 Feb;68(2):159-67.
PubMed
Byrareddy SN, Little D, Mayne AE, Villinger F, Ansari AA: Phenotypic and functional characterization of monoclonal antibodies with specificity for rhesus macaque CD200, CD200R and mincle. PLoS One. 2015 Oct 15;10(10):e0140689.
PubMed
Foster-Cuevas M, Wright GJ, Puklavec MJ, Brown MH, Barclay AN: Human herpesvirus 8 K14 protein mimics CD200 in down-regulating macrophage activation through CD200 receptor. J Virol. 2004 Jul;78(14):7667-76.
PubMed
Mihrshahi R, Barclay AN, Brown MH: Essential roles for Dok2 and RasGAP in CD200 receptor-mediated regulation of human myeloid cells. J Immunol. 2009 Oct 15;183(8):4879-86.
PubMed
Misstear K, Chanas SA, Rezaee SA, Colman R, Quinn LL, Long HM, Goodyear O, Lord JM, Hislop AD, Blackbourn DJ: Suppression of antigen-specific T cell responses by the Kaposi's sarcoma-associated herpesvirus viral OX2 protein and its cellular orthologue, CD200. J Virol. 2012 Jun;86(11):6246-57.
PubMed
Letarte M, Voulgaraki D, Hatherley D, Foster-Cuevas M, Saunders NJ, Barclay AN: Analysis of leukocyte membrane protein interactions using protein microarrays. BMC Biochem. 2005 Mar 1;6:2. doi: 10.1186/1471-2091-6-2.
PubMed
Variant
100 tests
A6-893-T100
In stock
242.00 USD
Variant
100 tests
A6-893-T100
In stock
242.00 USD

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