Anti-Hu CD200 APC

Anti-Hu CD200 APC
Regulatory status
RUO
Antigen
CD200
Clone
OX-104
Format
APC
Reactivity
Human
Application
Excitation laser
red (633 nm)
Variant
100 tests
1A-746-T100
In stock
242.00 USD

25 tests
1A-746-T025
In stock
121.00 USD
Variant
100 tests
1A-746-T100
In stock
242.00 USD

25 tests
1A-746-T025
In stock
121.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody OX-104 recognizes an extracellular epitope of CD200, a type-1 glycoprotein of the immunoglobulin superfamily, which is expressed in neurons, B and T cell subsets, keratinocytes, follicular dendritic cells, and ovarian cells.
Workshop
HLDA VII: WS Code 70655
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
recombinant human CD200
Preparation
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
MRC, MOX, OX-2
Antigen description
CD200 (also known as OX2 or MRC) is a type-1 membrane glycoprotein, which contains two extracellular immunoglobulin domains, transmembrane domain and cytoplasmic domain. It is expressed by neuronal cells, B and T cell subsets, follicular dendritic cells, keratinocytes, and ovarian cells. The interaction between CD200 and its receptor CD200R results in macrophage activation (IL-6 production), inhibition of mast cell degranulation along with reduced TNF-alpha and IL-13 secretion and overall attenuation of the activation status of lymphocytes. It seems CD200 is also involved in maternal tolerance and its decreased expression in hair follicle correlates with follicular miniaturization.
Entrez Gene ID 4345
UniProt ID P41217
1A-746 FC
Flow cytometry multicolor surface staining pattern of human lymfocytes stained using anti-human CD3 (UCHT1) PE antibody (20 μl reagent / 100 μl of peripheral whole blood) and anti-human CD200 (OX-104) APC antibody (concentration in sample 0.56 μg/ml).
1A-746_FC_Dot-plot
Flow cytometry multicolor surface staining pattern of human lymfocytes stained using anti-human CD19 (LT19) PE antibody (20 μl reagent / 100 μl of peripheral whole blood) and anti-human CD200 (OX-104) APC antibody (concentration in sample 0.56 μg/ml).
1A-746_FC_Histogram
Separation of human CD200 positive CD19 positive B cells (red-filled) from CD200 negative CD19 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD200 (OX-104) APC antibody (concentration in sample 0.56 μg/ml).
1A-746_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD200 (OX-104) APC antibody (concentration in sample 0.56 μg/ml).

Product specific references:

Garza LA, Yang CC, Zhao T, Blatt HB, Lee M, He H, Stanton DC, Carrasco L, Spiegel JH, Tobias JW, Cotsarelis G: Bald scalp in men with androgenetic alopecia retains hair follicle stem cells but lacks CD200-rich and CD34-positive hair follicle progenitor cells. J Clin Invest. 2011 Feb;121(2):613-22
PubMed
Li Y, Zhao LD, Tong LS, Qian SN, Ren Y, Zhang L, Ding X, Chen Y, Wang YX, Zhang W, Zeng XF, Zhang FC, Tang FL, Zhang X, Ba DN, He W, Cao XT, Lipsky PE: Aberrant CD200/CD200R1 expression and function in systemic lupus erythematosus contributes to abnormal T-cell responsiveness and dendritic cell activity. Arthritis Res Ther. 2012 May 23;14(3):R123. doi: 10.1186/ar3853.
PubMed
Sopp P, Werling D, Baldwin C: Sopp P, Werling D, Baldwin C: Vet Immunol Immunopathol. 2007 Sep 15;119(1-2):106-14
PubMed
Shiratori I, Yamaguchi M, Suzukawa M, Yamamoto K, Lanier LL, Saito T, Arase H: Down-regulation of basophil function by human CD200 and human herpesvirus-8 CD200. J Immunol. 2005 Oct 1;175(7):4441-9.
PubMed
Colmont CS, Benketah A, Reed SH, Hawk NV, Telford WG, Ohyama M, Udey MC, Yee CL, Vogel JC, Patel GK: CD200-expressing human basal cell carcinoma cells initiate tumor growth. Proc Natl Acad Sci U S A. 2013 Jan 22;110(4):1434-9.
PubMed
Foster-Cuevas M, Wright GJ, Puklavec MJ, Brown MH, Barclay AN: Human herpesvirus 8 K14 protein mimics CD200 in down-regulating macrophage activation through CD200 receptor. J Virol. 2004 Jul;78(14):7667-76.
PubMed
Meuth SG, Simon OJ, Grimm A, Melzer N, Herrmann AM, Spitzer P, Landgraf P, Wiendl H: CNS inflammation and neuronal degeneration is aggravated by impaired CD200-CD200R-mediated macrophage silencing. J Neuroimmunol. 2008 Feb;194(1-2):62-9.
PubMed
Variant
100 tests
1A-746-T100
In stock
242.00 USD

25 tests
1A-746-T025
In stock
121.00 USD
Variant
100 tests
1A-746-T100
In stock
242.00 USD

25 tests
1A-746-T025
In stock
121.00 USD

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