Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD2 PE

Anti-Hu CD2 PE

Regulatory status

RUO

Antigen

CD2

Clone

LT2

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-492-T100

In stock

242.00 USD

25 tests

1P-492-T025

Delivery 1 week

121.00 USD

Variant

100 tests

1P-492-T100

In stock

242.00 USD

25 tests

1P-492-T025

Delivery 1 week

121.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b

Specificity

The antibody LT2 reacts with an extracellular epitope of CD2, a 50 kDa glycoprotein present on the human peripheral blood T lymphocytes and NK cells; also expressed by all thymocytes.

Workshop

HLDA VI: WS Code T 6T-008

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

Normal human blood lymphocytes.

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

T11, LFA-2, SRBC

Antigen description

CD2 belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interaction with CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection.

Entrez Gene ID 914

UniProt ID P06729

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD2 (LT2) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD2 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD2 (LT2) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).

Separation of MOLT-4 cells stained using anti-human CD2 (LT2) PE antibody (concentration in sample 4 μg/m, red-filled) from MOLT-4 cells stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 4 μg/ml, same as CD2 PE concentration, black-dashed) in flow cytometry analysis (surface staining).

General references:

Pawlowski NN, Struck D, Grollich K, Kuhl AA, Zeitz M, Liesenfeld O, Hoffmann JC: CD2 deficiency partially prevents small bowel inflammation and improves parasite control in murine Toxoplasma gondii infection. World J Gastroenterol. 2007 Aug 21;13(31):4207-13.
PubMed

Zhu DM, Dustin ML, Cairo CW, Thatte HS, Golan DE: Mechanisms of cellular avidity regulation in CD2-CD58-mediated T cell adhesion. ACS Chem Biol. 2006 Nov 21;1(10):649-58.
PubMed

Dustin ML: Adhesive bond dynamics in contacts between T lymphocytes and glass-supported planar bilayers reconstituted with the immunoglobulin-related adhesion molecule CD58. J Biol Chem. 1997 Jun 20;272(25):15782-8.
PubMed

Product specific references:

Filatov AV, Bachurin PS, Markova NA, Surnakova NE: [A panel of monoclonal antibodies against human lymphocyte antigens] Eksp Onkol. 1989;11(2):28-32.
PubMed

Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).

Gannon GA, Rhind SG, Suzui M, Zamecnik J, Sabiston BH, Shek PN, Shephard RJ: beta-Endorphin and natural killer cell cytolytic activity during prolonged exercise. Is there a connection? Am J Physiol. 1998 Dec;275(6 Pt 2):R1725-34.
PubMed

Lin CW, Liu TY, Chen SU, Wang KT, Medeiros LJ, Hsu SM: CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features. Blood. 2005 Nov 15;106(10):3567-74.
PubMed