Anti-Hu CD2 Alexa Fluor® 700

Anti-Hu CD2 Alexa Fluor<sup>®</sup> 700
Regulatory status
RUO
Antigen
CD2
Clone
LT2
Format
Alexa Fluor® 700
Reactivity
Human
Application
Excitation laser
red (633 nm)
Variant
100 tests
A7-492-T100
In stock
242.00 USD
Variant
100 tests
A7-492-T100
In stock
242.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG2b
Specificity
The antibody LT2 reacts with an extracellular epitope of CD2, a 50 kDa glycoprotein present on the human peripheral blood T lymphocytes and NK cells; also expressed by all thymocytes.
Workshop
HLDA VI: WS Code T 6T-008
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Normal human blood lymphocytes.
Preparation
Purified antibody is conjugated with Alexa Fluor® 700 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Other names
T11, LFA-2, SRBC
Antigen description
CD2 belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interaction with CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection.
Entrez Gene ID 914
UniProt ID P06729
A7-492_FC_Profil značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD2 (LT2) Alexa Fluor® 700 antibody (4 μl reagent / 100 μl of peripheral whole blood).
A7-492_FC_Histogram
Separation of human CD2 positive lymphocytes (red-filled) from CD2 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD2 (LT2) Alexa Fluor® 700 antibody (4 μl reagent / 100 μl of peripheral whole blood).

General references:

Pawlowski NN, Struck D, Grollich K, Kuhl AA, Zeitz M, Liesenfeld O, Hoffmann JC: CD2 deficiency partially prevents small bowel inflammation and improves parasite control in murine Toxoplasma gondii infection. World J Gastroenterol. 2007 Aug 21;13(31):4207-13.
PubMed
Zhu DM, Dustin ML, Cairo CW, Thatte HS, Golan DE: Mechanisms of cellular avidity regulation in CD2-CD58-mediated T cell adhesion. ACS Chem Biol. 2006 Nov 21;1(10):649-58.
PubMed
Dustin ML: Adhesive bond dynamics in contacts between T lymphocytes and glass-supported planar bilayers reconstituted with the immunoglobulin-related adhesion molecule CD58. J Biol Chem. 1997 Jun 20;272(25):15782-8.
PubMed
Pawlowski NN, Struck D, Grollich K, Kuhl AA, Zeitz M, Liesenfeld O, Hoffmann JC: CD2 deficiency partially prevents small bowel inflammation and improves parasite control in murine Toxoplasma gondii infection. World J Gastroenterol. 2007 Aug 21;13(31):4207-13.
PubMed

Product specific references:

Filatov AV, Bachurin PS, Markova NA, Surnakova NE: [A panel of monoclonal antibodies against human lymphocyte antigens] Eksp Onkol. 1989;11(2):28-32.
PubMed
Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
Gannon GA, Rhind SG, Suzui M, Zamecnik J, Sabiston BH, Shek PN, Shephard RJ: beta-Endorphin and natural killer cell cytolytic activity during prolonged exercise. Is there a connection? Am J Physiol. 1998 Dec;275(6 Pt 2):R1725-34.
PubMed
Lin CW, Liu TY, Chen SU, Wang KT, Medeiros LJ, Hsu SM: CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features. Blood. 2005 Nov 15;106(10):3567-74.
PubMed
Variant
100 tests
A7-492-T100
In stock
242.00 USD
Variant
100 tests
A7-492-T100
In stock
242.00 USD

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